Wide-Inhibitory Spectra Bacteriocins Produced by Lactobacillus gasseri K7

Probiotics and Antimicrobial Proteins
Metoda Zorič PeternelIrena Rogelj

Abstract

The aim of our study was to determine the genetic characterization and classification of Lb. gasseri K7 bacteriocins, comparison with bacteriocins of the Lb. gasseri LF221 strain and other related strains. Bacteriocin-encoding genes were amplified by PCR, subjected to DNA sequencing, and BLAST sequence analysis was performed to search the database for homologous peptides. Lb. gasseri K7 produces two two-peptide bacteriocins, named gassericin K7 A and gassericin K7 B. Their nucleotide sequences were deposited at GenBank, under accession numbers EF392861 for the gassericin K7 A and AY307382 for the gassericin K7 B. Analysis of gene clusters of bacteriocins in Lb. gasseri K7 strain revealed a 100 percent sequence identity with bacteriocins in LF221 strain. An active peptide of gassericin K7 B is homologous to the complementary peptide of gassericin T, and a complementary peptide of gassericin K7 B is homologous to the active peptide of gassericin T. Another surprising finding was that the sakacin T-beta peptide is partly homologous to the active peptide of gassericin K7 A, while the other sakacin T peptide (alfa) is partly homologous to the complementary peptide of gassericin K7 B. Gassericins of Lb. gasseri K7 strain were both cl...Continue Reading

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Citations

Nov 9, 2012·Folia Microbiologica·Kristýna TurkováBojana Bogovič Matijašić
Jul 26, 2014·Genome Announcements·Primož TrevenIrena Rogelj
Aug 18, 2020·Applied and Environmental Microbiology·Trine NilsenThomas P Parks

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