Wide-Ranging Analysis of MicroRNA Profiles in Sporadic Amyotrophic Lateral Sclerosis Using Next-Generation Sequencing

Frontiers in Genetics
Bruna De FeliceAntonio Federico

Abstract

MicroRNA (miRNA) has emerged as an important regulator of gene expression in neurodegenerative disease as amyotrophic lateral sclerosis (ALS). In the nervous system, dysregulation in miRNA-related pathways is subordinated to neuronal damage and cell death, which contributes to the expansion of neurodegenerative disorders, such as ALS. In the present research, we aimed to profile dysregulation of miRNAs in ALS blood and neuromuscular junction as well as healthy blood control by next-generation sequencing (NGS). The expression of three upregulated miRNAs, as miR-338-3p, miR-223-3p, and miR-326, in the ALS samples compared to healthy controls, has been validated by qRT-PCR in a cohort of 45 samples collected previously. Bioinformatics tools were used to perform ALS miRNAs target analysis and to predict novel miRNAs secondary structure. The analysis of the NGS data identified 696 and 49 novel miRNAs which were differentially expressed in ALS tissues. In particular, in neuromuscular junction the differential expression of miR-338-3p, which we previously found upregulated in different types of ASL tissues, miR-223-3p, and miR-326 was elevated compared to normal control. ALS miRNAs gene target were significantly involved in neuronal r...Continue Reading

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Citations

Jan 7, 2019·Molecular Neurobiology·Kinga CzubowiczRobert P Strosznajder
Oct 3, 2020·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Lin Wang, Lijuan Zhang
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Aug 2, 2020·Molecular Neurobiology·Bruna De FeliceCinzia Coppola
Jun 28, 2019·Frontiers in Neurology·Sophie FogginRob Layfield
Mar 7, 2020·International Journal of Molecular Sciences·Metka Ravnik-Glavač, Damjan Glavač
Feb 20, 2021·Metabolic Brain Disease·Nader Akbari DilmaghaniSoudeh Ghafouri-Fard

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Datasets Mentioned

BETA
E-MTAB-7073
1

Methods Mentioned

BETA
RNA-Seq
PCR
Chip
GTPase

Software Mentioned

OpenTarget
R scripts
TargetScan
iMir
Bioconductor package
NOISeq
Cutadapt
ArrayExpress
Bowtie
RNAstructure

Related Concepts

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Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.