Widely targeted metabolomics of Alzheimer's disease postmortem cerebrospinal fluid based on 9-fluorenylmethyl chloroformate derivatized ultra-high performance liquid chromatography tandem mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Yoshio MugurumaKoichi Inoue

Abstract

Confirmed biomarkers of postmortem cerebrospinal fluid (pCSF) are used to differentiate between Alzheimer's disease (AD) patients and healthy seniors with high diagnostic accuracy. However, the extent to which the performance of specific metabolic profiling facilitates reliable estimations of the concentrations of the different pCSF biomarkers and their ratios remains unclear. The interpretation of the lower levels of molecules of metabolic profiling and their concentration ratios in pCSF related to brain disorders could facilitate an unchallenging detection of peripheral biomarkers of AD stages and other dementia types. In this study, we proposed the use of widely targeted metabolomics for pCSF metabolic profiling using 9-fluorenylmethyl chloroformate- (FMOC) derivatized ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to evaluate the diversity of 97 amine-mediated metabolic patterns and pathways from confirmed diagnosis based on AD brain pathology. Our results identified the metabolites that contributed toward and mutually influenced the principal component analysis plot with integrated analytes. Furthermore, the AD group showed a significant variation in several analyte concentration levels...Continue Reading

Citations

Dec 26, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yukiko HorieTatsuya Higashi
Dec 18, 2019·Bioanalysis·Karen SegersAnn Van Eeckhaut
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·David J BealeDaniel A Dias
Sep 12, 2019·Frontiers in Pharmacology·Knut BiberThomas Möller
Oct 22, 2020·Biomedical Chromatography : BMC·Victor DavidToma Galaon
Aug 29, 2021·Metabolomics : Official Journal of the Metabolomic Society·Pierluigi RevegliaGaetano Corso
Jan 7, 2022·Metabolomics : Official Journal of the Metabolomic Society·George E JaskiwCurtis J Donskey

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