Widespread Cell-Specific Prolactin Receptor Expression in Multiple Murine Organs

Endocrinology
Mari AokiUlrich Boehm

Abstract

The prolactin receptor (Prlr) mediates not only the multiple effects of prolactin, but also those of the placental lactogens and, in humans, some actions of growth hormone. Although Prlr expression has been reported to be widespread in the body, specific cellular expression patterns within tissues are undefined for many organs. One persisting problem in investigating Prlr function is that the protein is difficult to detect using conventional methods. To allow investigation of Prlr expression with a single cell resolution, we have recently developed a knock-in mouse strain in which Cre recombinase is expressed together with the long isoform of the Prlr using an internal ribosome entry site. When crossed to a Cre-dependent reporter mouse strain, Cre-mediated recombination will genetically label cells that acutely express the Prlr as well as cells that have transiently expressed the Prlr during development. We report here the anatomical distribution of cells which express the fluorescent reporter τ green fluorescent protein in a total of 38 organs prepared from young adult male and female Prlr reporter mice. Our results establish a resource for dissecting the functional role of Prlr in multiple murine tissues.

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Citations

Oct 2, 2020·Journal of Neuroendocrinology·Papillon GustafsonDavid R Grattan
Jul 3, 2020·Frontiers in Neuroscience·Arpád DobolyiMelinda Cservenák
Oct 10, 2020·Journal of Neuroendocrinology·Teodora GeorgescuDavid R Grattan
Nov 19, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hui LiAmanda J Page
Jan 6, 2021·Communications Biology·Susana ValenteSusana Q Lima
Jan 20, 2021·Journal of Neuroendocrinology·Felicitas Lopez-VicchiDamasia Becu-Villalobos
Mar 27, 2021·Frontiers in Neuroendocrinology·G Molina-SalinasM Cerbon
Apr 20, 2021·General and Comparative Endocrinology·Pamela ParéMaria Cátira Bortolini
Oct 17, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Cosmin I Ciotu, Michael J M Fischer

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