Widespread Distribution and Functional Specificity of the Copper Importer CcoA: Distinct Cu Uptake Routes for Bacterial Cytochrome c Oxidases

MBio
Bahia Khalfaoui-HassaniFevzi Daldal

Abstract

Cytochrome c oxidases are members of the heme-copper oxidase superfamily. These enzymes have different subunits, cofactors, and primary electron acceptors, yet they all contain identical heme-copper (CuB) binuclear centers within their catalytic subunits. The uptake and delivery pathways of the CuB atom incorporated into this active site, where oxygen is reduced to water, are not well understood. Our previous work with the facultative phototrophic bacterium Rhodobacter capsulatus indicated that the copper atom needed for the CuB site of cbb3-type cytochrome c oxidase (cbb3-Cox) is imported to the cytoplasm by a major facilitator superfamily-type transporter, CcoA. In this study, a comparative genomic analysis of CcoA orthologs in alphaproteobacterial genomes showed that CcoA is widespread among organisms and frequently co-occurs with cytochrome c oxidases. To define the specificity of CcoA activity, we investigated its function in Rhodobacter sphaeroides, a close relative of R. capsulatus that contains both cbb3- and aa3-Cox. Phenotypic, genetic, and biochemical characterization of mutants lacking CcoA showed that in its absence, or even in the presence of its bypass suppressors, only the production of cbb3-Cox and not that of ...Continue Reading

References

Apr 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·B MarrsH Gest
Sep 1, 1994·Journal of Bacteriology·J A García-HorsmanR B Gennis
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·H MichelA Kannt
Aug 26, 1998·Biochimica Et Biophysica Acta·M Toledo-CuevasJ A García-Horsman
Jan 5, 2000·The Journal of Biological Chemistry·L HiserJ P Hosler
May 19, 2000·The Journal of Biological Chemistry·T V O'Halloran, V C Culotta
May 4, 2001·Biochimica Et Biophysica Acta·M M PereiraM Teixeira
Jun 14, 2002·The Journal of Biological Chemistry·Heather S CarrDennis R Winge
Apr 26, 2003·Trends in Biochemical Sciences·Thijs J G EttemaJohn van der Oost
Jun 1, 1960·Journal of General Microbiology·W R SISTROM
Jan 20, 2004·The Journal of Biological Chemistry·Paul A CobineDennis R Winge
Jul 2, 2004·Human Molecular Genetics·Scot C LearyEric A Shoubridge
Mar 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lucia BanciStefano Mangani
Oct 11, 2005·Nucleic Acids Research·Ross OverbeekVeronika Vonstein
Nov 18, 2005·Journal of Bacteriology·Danielle L SwemCarl E Bauer
Nov 11, 2006·Annual Review of Genetics·Richard MoxonDerek Hood
Mar 21, 2008·Molecular Biology and Evolution·Anne-Lise DucluzeauWolfgang Nitschke
Jun 3, 2008·Biochimica Et Biophysica Acta·Manuela M PereiraMiguel Teixeira
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Lee Macomber, James A Imlay
Oct 29, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ya Hui HungRobert Alan Cherny
Mar 13, 2010·PloS One·Morgan N PriceAdam P Arkin
Apr 7, 2010·The Journal of Biological Chemistry·Bahia Khalfaoui HassaniSoufian Ouchane
Jun 26, 2010·Science·Sabine BuschmannHartmut Michel
Nov 26, 2010·Molecular Microbiology·Manuel González-GuerreroJosé M Argüello
Jul 1, 2011·Nature·Pontus GourdonPoul Nissen
Nov 15, 2011·Biochimica Et Biophysica Acta·Seda EkiciFevzi Daldal
Nov 30, 2011·Nucleic Acids Research·Wei-Hsiang Lin, Edo Kussell
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Jan 29, 2013·Nature Chemical Biology·Yue FuDavid P Giedroc
Aug 10, 2013·FEMS Microbiology Reviews·Kai ZhouChris W Michiels
Nov 22, 2013·Biochemical Society Transactions·Jude BeaudoinSimon Labbé
Dec 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Crysten E Blaby-HaasSabeeha S Merchant

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Citations

Oct 20, 2018·Metallomics : Integrated Biometal Science·Louisa J StewartKarrera Y Djoko
Oct 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Dorian MarckmannHans-Georg Koch
Mar 10, 2020·Metallomics : Integrated Biometal Science·Nur SelamogluFevzi Daldal
Apr 25, 2020·Molecular Microbiology·Andrea Giachino, Kevin J Waldron
May 3, 2020·Applied and Environmental Microbiology·Shreya SrivastavaBrandon R Briggs
Sep 24, 2020·Membranes·Andreea AndreiHans-Georg Koch
Feb 6, 2019·Scientific Reports·Yang ZhangBahia Khalfaoui-Hassani
Oct 15, 2019·Frontiers in Microbiology·Rudy AntoineFrançoise Jacob-Dubuisson
Nov 12, 2021·PLoS Biology·Satya Prathyusha BhamidimarriBert van den Berg

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Methods Mentioned

BETA
phylogenetic profile

Software Mentioned

MAFFT
CIPRES
XSEDE
FastTreeMP
yFiles
Cytoscape
Interactive Tree of Life ( iTOL )

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