Mar 30, 2020

SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction

BioRxiv : the Preprint Server for Biology
Juna Carlevaro-FitaGonzalo P. Barriga

Abstract

The ongoing COVID-19 pandemic has reached more than 200 countries and territories worldwide. Given the large requirement of SARS-CoV-2 diagnosis and considering that RNA extraction kits are in short supply, we investigated whether two commercial RT-qPCR kits were compatible with direct SARS-CoV-2 detection from nasopharyngeal swab samples. We show that one of the tested kits is fully compatible with direct SARS-CoV-2 detection suggesting that omission of RNA extraction step should be considered in SARS-CoV-2 diagnosis.

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Mentioned in this Paper

Polyribosomes
Establishment and Maintenance of Localization
Study
Transcriptional Regulation
CFC1 gene
Set of Polysomal Ribosomes
Cap Formation
Exons
Human Cell Line
Cytoplasmic

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