Widespread Myocardial Delivery of Heart-Derived Stem Cells by Nonocclusive Triple-Vessel Intracoronary Infusion in Porcine Ischemic Cardiomyopathy: Superior Attenuation of Adverse Remodeling Documented by Magnetic Resonance Imaging and Histology

PloS One
Eleni TseliouEduardo Marbán

Abstract

Single-vessel, intracoronary infusion of stem cells under stop-flow conditions has proven safe but achieves only limited myocardial coverage. Continuous flow intracoronary delivery to one or more coronary vessels may achieve broader coverage for treating cardiomyopathy, but has not been investigated. Using nonocclusive coronary guiding catheters, we infused allogeneic cardiosphere-derived cells (CDCs) either in a single vessel or sequentially in all three coronary arteries in porcine ischemic cardiomyopathy and used magnetic resonance imaging (MRI) to assess structural and physiological outcomes. Vehicle-infused animals served as controls. Single-vessel stop-flow and continuous-flow intracoronary infusion revealed equivalent effects on scar size and function. Sequential infusion into each of the three major coronary vessels under stop-flow or continuous-flow conditions revealed equal efficacy, but less elevation of necrotic biomarkers with continuous-flow delivery. In addition, multi-vessel delivery resulted in enhanced global and regional tissue function compared to a triple-vessel placebo-treated group. The functional benefits after global cell infusion were accompanied histologically by minimal inflammatory cellular infiltra...Continue Reading

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Citations

Jan 26, 2017·Heart, Lung & Circulation·Hai-Ying ChenLe-Xin Wang
Jul 5, 2017·Circulation·David J Lefer, Eduardo Marbán
Dec 6, 2017·Cardiology in Review·Carmel Ashur, William H Frishman
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Feb 12, 2019·Nature Biomedical Engineering·Eduardo Marbán
Feb 16, 2021·Frontiers in Physiology·Wei ZhangQuanjin Si
Dec 11, 2020·Science Translational Medicine·Kenta HiraiHidemasa Oh
Aug 20, 2021·Current Cardiology Reports·Devika KirMrudula R Munagala

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Methods Mentioned

BETA
light microscopy

Software Mentioned

GraphPad Prism
ImageJ
Las AF Lite
Image J
harmonic phase ( HARP
DYNAMIC

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