PMID: 11906454Mar 22, 2002Paper

Wild-type intracellular bacteria deliver DNA into mammalian cells

Cellular Microbiology
C Grillot-CourvalinP Courvalin

Abstract

Gene transfer in vitro from intracellular bacteria to mammalian phagocytic and non-phagocytic cells and in vivo in mice has been reported. The bacteria used as DNA delivery vectors were engineered to lyze upon entry in the cell due to impaired cell wall synthesis for Shigella flexneri and invasive Escherichia coli, or production of a phage lysin for Listeria mono- cytogenes. In vivo gene transfer was obtained with attenuated Salmonella typhimurium and resulted in stimulation of mucosal immunity. We report that wild-type intracellular human pathogens, such as L. monocytogenes EGD or LO28 and S. flexneri M90T, mediate efficient in vitro transfer of functional genes into epithelial and macrophage cell lines. A low- efficiency transfer was obtained from strain EGD to mouse peritoneal macrophages. DNA transfer with S. typhimurium was observed only from atten-uated aroA strain SL7207 into COS-1 cell line. As demonstrated by the study of listeriolysin-defective L. monocytogenes or of S. typhimurium SL7207 aroA engineered to secrete listeriolysin, escape of bacteria or of plasmid DNA from the intracytoplasmic vacuole is required for transfer of genetic information to occur.

References

Jul 9, 1992·Nature·A ZychlinskyP J Sansonetti
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S G GrantD Hanahan
Jul 1, 1986·Annales De L'Institut Pasteur. Microbiology·P PardonI Miras
Mar 1, 1982·Infection and Immunity·P J SansonettiS B Formal
Sep 1, 1995·Infection and Immunity·C A GuzmanK N Timmis
Oct 1, 1995·Trends in Microbiology·F Garcia-del Portillo, B B Finlay
Jul 1, 1997·Infection·S SchlechtC Galanos
Sep 22, 1998·Nature Biotechnology·C Grillot-CourvalinP Courvalin
Jul 16, 1999·The EMBO Journal·K UchiyaE A Groisman
Oct 6, 1999·Current Opinion in Biotechnology·C Grillot-CourvalinP Courvalin
Mar 23, 2000·FEMS Immunology and Medical Microbiology·A DarjiS Weiss
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium

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Citations

Jun 5, 2003·International Journal for Parasitology·Guido DietrichIvaylo Gentschev
Apr 9, 2005·Gene Therapy·I CastagliuoloG Palù
Jun 24, 2005·Gene Therapy·A LanerC Grillot-Courvalin
May 11, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Nelita ElliottFan Yuan
Mar 30, 2006·DNA and Cell Biology·N C SoudersY Paterson
Jan 29, 2010·Human Gene Therapy·Jan Peter van PijkerenMark Tangney
Sep 24, 2010·Journal of Biomedicine & Biotechnology·Sara Pérez-Luz, Javier Díaz-Nido
Apr 12, 2008·BMC Cancer·Jochen StritzkerWerner Goebel
Jan 8, 2009·Journal of Immune Based Therapies and Vaccines·Jerome S HarmsGary A Splitter
Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Charles H JonesBlaine A Pfeifer
May 29, 2010·Journal of Biomedicine & Biotechnology·Toshi Nagata, Yukio Koide
Jun 19, 2004·Journal of Drug Targeting·F Xu, J B Ulmer
Mar 5, 2004·Expert Opinion on Biological Therapy·Holger Loessner, Siegfried Weiss
Aug 21, 2015·Research in Microbiology·Katsunori SuzukiShinji Yamamoto
Oct 8, 2004·Cellular Microbiology·Liljana PetrovskaGordon Dougan
Apr 21, 2005·Cellular Microbiology·Christoph SchoenWerner Goebel
Apr 28, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Christopher Bazinet
Sep 21, 2006·Cellular Microbiology·Matthew J GiacaloneRoger A Sabbadini
Dec 20, 2005·The Journal of Pathology·Georges VassauxNick R Lemoine
Sep 1, 2008·Microbial Biotechnology·Sukhadeo Barbuddhe, Trinad Chakraborty
Oct 7, 2006·Biotechnology Progress·Benjamin E SimonRonald A Barry
Mar 25, 2010·European Journal of Immunology·Sarah KimVeit Hornung
Jul 11, 2006·Vaccine·Gordon Dougan, Carlos Hormaeche
May 5, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G van den EedeA Wilcks
May 21, 2003·International Journal of Medical Microbiology : IJMM·Siegfried Weiss
Nov 10, 2004·International Journal of Medical Microbiology : IJMM·Christoph SchoenSabine Pilgrim
Jan 28, 2015·Molecular Pharmaceutics·Charles H JonesBlaine A Pfeifer
Oct 31, 2016·Advanced Drug Delivery Reviews·Zeinab HosseinidoustMetin Sitti
Sep 20, 2008·Environmental Biosafety Research·Paul Keese
Jan 1, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Charles H JonesBlaine A Pfeifer
Nov 3, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Michael FöllerFlorian Lang
Dec 17, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karolin MeixenbergerBastian Opitz
May 28, 2019·Microbiology Spectrum·Sarah E F D'Orazio
Jan 20, 2007·Molecular Pharmaceutics·Saba Parsa, Blaine Pfeifer

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