Abstract
Skeletal muscle is formed via a complex series of events during embryogenesis. These events include commitment of mesodermal precursor cells, cell migration, cell-cell recognition, fusion of myoblasts, activation of structural genes, and maturation. In mice lacking the bHLH transcription factor myogenin, myoblasts are specified and positioned correctly, but few fuse to form multinucleated fibers. This indicates that myogenin is critical for the fusion process and subsequent differentiation events of myogenesis. To further define the nature of the myogenic defects in myogenin-null mice, we investigated whether myogenin-null myoblasts are capable of fusing with wild-type myoblasts in vivo using chimeric mice containing mixtures of myogenin-null and wild-type cells. Chimeric embryos demonstrated that myogenin-null myoblasts readily fused in the presence of wild-type myoblasts. However, chimeric myofibers did not express wild-type levels of muscle-specific gene products, and myofibers with a high percentage of mutant nuclei appeared abnormal, suggesting that the wild-type nuclei could not fully rescue mutant nuclei in the myofibers. These data demonstrate that myoblast fusion can be uncoupled from complete myogenic differentiation ...Continue Reading
References
Apr 1, 1992·Trends in Genetics : TIG·M Buckingham
Sep 1, 1991·Genes & Development·G Friedrich, P Soriano
Sep 1, 1990·Genes & Development·E N Olson
Feb 9, 1989·Nature·G K PavlathH M Blau
Apr 10, 1987·Nucleic Acids Research·J A Simon, J T Lis
Feb 1, 1995·The Journal of Cell Biology·J M VenutiW H Klein
Oct 19, 1995·Nature·T Yagami-HiromasaA Fujisawa-Sehara
Nov 1, 1995·Developmental Biology·A RawlsE N Olson
May 1, 1995·Anatomy and Embryology·B Christ, C P Ordahl
Mar 1, 1993·General and Comparative Endocrinology·D C McFarlandK K Gilkerson
May 19, 1995·The Journal of Biological Chemistry·M E ColemanR J Schwartz
Jun 1, 1995·Genes & Development·W ZhangE N Olson
Jan 1, 1995·Developmental Biology·H M Stern, S D Hauschka
Jun 1, 1994·Current Opinion in Cell Biology·A Lassar, A Münsterberg
Jan 1, 1994·Genes & Development·E N Olson, W H Klein
Aug 5, 1993·Nature·P HastyW H Klein
Aug 5, 1993·Nature·Y NabeshimaY Nabeshima
Aug 1, 1993·Endocrinology·K M RosenL Villa-Komaroff
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S M HollenbergH Weintraub
Apr 5, 1996·Cell·E N OlsonB J Wold
Oct 1, 1996·The Journal of Cell Biology·J C EngertN Rosenthal
Citations
May 18, 2000·Biochemical and Biophysical Research Communications·W J ChoW K Lim
Jul 27, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P Y Rescan
May 15, 2009·The Journal of Biological Chemistry·Tetsuaki MiyakeJohn C McDermott
Jan 12, 2012·PloS One·Nikolaos P MastroyiannopoulosLeonidas A Phylactou
May 15, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C F Shuler, K R Dalrymple
Nov 24, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·N NicolasC Chanoine
Jan 1, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christophe Chanoine, Serge Hardy
Jul 15, 2015·PloS One·Sebastian FreseReiner Strick
Oct 23, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christophe ChanoineFrédéric Charbonnier
Oct 31, 2001·The Journal of Biological Chemistry·Frederic CharbonnierChristophe Chanoine
Dec 20, 2003·Endocrinology·Espen E SpangenburgFrank W Booth
Oct 14, 2018·Nature Communications·Massimo GanassiSimon M Hughes
Jan 13, 2006·Development·Jennifer R KnappWilliam H Klein
Feb 24, 2001·Developmental Biology·A MyerW H Klein
Sep 19, 1998·Developmental Biology·J RohwedelA M Wobus
Aug 30, 2008·Developmental Biology·Eric MeadowsWilliam H Klein