Wild-type myoblasts rescue the ability of myogenin-null myoblasts to fuse in vivo

Developmental Biology
A MyerW H Klein

Abstract

Skeletal muscle is formed via a complex series of events during embryogenesis. These events include commitment of mesodermal precursor cells, cell migration, cell-cell recognition, fusion of myoblasts, activation of structural genes, and maturation. In mice lacking the bHLH transcription factor myogenin, myoblasts are specified and positioned correctly, but few fuse to form multinucleated fibers. This indicates that myogenin is critical for the fusion process and subsequent differentiation events of myogenesis. To further define the nature of the myogenic defects in myogenin-null mice, we investigated whether myogenin-null myoblasts are capable of fusing with wild-type myoblasts in vivo using chimeric mice containing mixtures of myogenin-null and wild-type cells. Chimeric embryos demonstrated that myogenin-null myoblasts readily fused in the presence of wild-type myoblasts. However, chimeric myofibers did not express wild-type levels of muscle-specific gene products, and myofibers with a high percentage of mutant nuclei appeared abnormal, suggesting that the wild-type nuclei could not fully rescue mutant nuclei in the myofibers. These data demonstrate that myoblast fusion can be uncoupled from complete myogenic differentiation ...Continue Reading

References

Apr 1, 1992·Trends in Genetics : TIG·M Buckingham
Sep 1, 1990·Genes & Development·E N Olson
Feb 9, 1989·Nature·G K PavlathH M Blau
Feb 1, 1995·The Journal of Cell Biology·J M VenutiW H Klein
Oct 19, 1995·Nature·T Yagami-HiromasaA Fujisawa-Sehara
May 1, 1995·Anatomy and Embryology·B Christ, C P Ordahl
Mar 1, 1993·General and Comparative Endocrinology·D C McFarlandK K Gilkerson
Jan 1, 1995·Developmental Biology·H M Stern, S D Hauschka
Jun 1, 1994·Current Opinion in Cell Biology·A Lassar, A Münsterberg
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S M HollenbergH Weintraub
Oct 1, 1996·The Journal of Cell Biology·J C EngertN Rosenthal

❮ Previous
Next ❯

Citations

May 18, 2000·Biochemical and Biophysical Research Communications·W J ChoW K Lim
Jul 27, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P Y Rescan
May 15, 2009·The Journal of Biological Chemistry·Tetsuaki MiyakeJohn C McDermott
Jan 12, 2012·PloS One·Nikolaos P MastroyiannopoulosLeonidas A Phylactou
May 15, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C F Shuler, K R Dalrymple
Nov 24, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·N NicolasC Chanoine
Jan 1, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christophe Chanoine, Serge Hardy
Oct 23, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christophe ChanoineFrédéric Charbonnier
Oct 14, 2018·Nature Communications·Massimo GanassiSimon M Hughes
Aug 30, 2008·Developmental Biology·Eric MeadowsWilliam H Klein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.