Wilms' tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90

Cell Communication and Signaling : CCS
Yue KuaiRen Zhou

Abstract

Wilms' tumor 1-associating protein (WTAP) is a nuclear protein, which is ubiquitously expressed in many tissues. Furthermore, in various types of malignancies WTAP is overexpressed and plays a role as an oncogene. The function of WTAP in diffuse large B-cell lymphoma (DLBCL), however, remains unclear. Immunohistochemistry was applied to evaluate the levels of WTAP expression in DLBCL tissues and normal lymphoid tissues. Overexpression and knock-down of WTAP in DLBCL cell lines, verified on mRNA and protein level served to analyze cell proliferation and apoptosis in DLBCL cell lines by flow cytometry. Finally, co-immunoprecipitation (Co-IP), IP, and GST-pull down assessed the interaction of WTAP with Heat shock protein 90 (Hsp90) and B-cell lymphoma 6 (BCL6) as well as determined the extend of its ubiquitinylation. WTAP protein levels were consistently upregulated in DLBCL tissues. WTAP promoted DLBCL cell proliferation and improved the ability to confront apoptosis, while knockdown of WTAP in DLBCL cell lines allowed a significant higher apoptosis rate after treatment with Etoposide, an anti-tumor drug. The stable expression of WTAP was depended on Hsp90. In line, we demonstrated that WTAP could form a complex with BCL6 via Hsp...Continue Reading

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Citations

May 24, 2019·PloS One·Nuchjira TakheawWatchara Kasinrerk
Feb 10, 2021·Cell Biology International·Qin LiZhao Ma
Feb 23, 2021·Molecular Therapy. Nucleic Acids·Jiali HuangZhaoxia Wang

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Methods Mentioned

BETA
PCR
electrophoresis
flow cytometry
confocal microscopy
pull-down
ubiquitination
immunoprecipitation
immunoprecipitations
Co-IPs
transfection

Software Mentioned

Graph Pad Prism

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