PMID: 9445020Jan 28, 1998Paper

WIN 52035-dependent human rhinovirus 16: assembly deficiency caused by mutations near the canyon surface

Journal of Virology
W WangR R Rueckert

Abstract

Three drug-dependent mutants of human rhinovirus 16 (HRV16) were characterized by sequence analyses of spontaneous mutant isolates and were genetically reconstructed from a parental cDNA plasmid. These mutants formed plaques in the presence but not in the absence of the selecting antiviral drug, WIN 52035, which binds to the capsid of wild-type virus and inhibits its attachment to the host cell. The drug-dependent phenotype of each mutant was caused by a single amino acid substitution in the VP1 coat protein. The three independent mutations conferring drug dependence are M1103T, T1208A, and V1210A. Single-step growth experiments involving rescue of one of the three mutants (V1210A) by delayed drug addition suggested (i) that the drug dependence lesion is at the stage of virus assembly and (ii) that one or more components of the viral assembly pool decay in the absence of drug. RNA accumulation and infectivity were unaffected by the absence of drug in all three mutants, suggesting that the labile assembly component is coat protein.

References

Jan 1, 1976·The Journal of Infectious Diseases·D J D'AlessioE C Dick
Feb 1, 1978·The Journal of Allergy and Clinical Immunology·R K BushC E Reed
Jan 1, 1989·The Journal of Clinical Investigation·R F LemanskeW W Busse
Mar 10, 1989·Cell·J M GreveA McClelland
Apr 1, 1989·Antimicrobial Agents and Chemotherapy·W al-NakibP A Janssen
Nov 5, 1989·Journal of Molecular Biology·S S KimM A McKinlay
Sep 1, 1987·Virology·H ZeichhardtK O Habermehl
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R J ColonnoM A Murcko
Jan 1, 1985·Journal of Virology·B Sherry, R Rueckert
Sep 27, 1985·Science·J M HogleD J Filman
Jul 1, 1986·Antimicrobial Agents and Chemotherapy·M P FoxM A McKinlay
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
May 1, 1971·Virology·K C MedappaR R Rueckert
Aug 1, 1984·The Journal of Infectious Diseases·C K MeschievitzE C Dick
Sep 15, 1993·Structure·M A OliveiraM A McKinlay
Apr 1, 1993·Journal of Virology·D A ShepardR R Rueckert
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T J SmithT S Baker
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·V R Racaniello

❮ Previous
Next ❯

Citations

Sep 28, 2004·The Journal of General Virology·S P AminevaJ E Gern
Apr 3, 2008·Medicinal Research Reviews·Armando M De PalmaJohan Neyts
May 28, 2015·Antimicrobial Agents and Chemotherapy·Lise SchotteJames M Hogle
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·J W Drake, J J Holland
Nov 4, 2008·American Journal of Respiratory Cell and Molecular Biology·Lingxiang ZhuYin Chen
Dec 30, 2011·American Journal of Respiratory Cell and Molecular Biology·Shasha TaoYin Chen
Jun 4, 1999·Journal of Clinical Pharmacology·S M Abdel-Rahman, G L Kearns
Apr 13, 2007·Journal of Virology·Umesh Katpally, Thomas J Smith
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·David J HallPaul J Bertics
Jul 23, 2005·Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders : Official Publication of the World Federation of Neurology, Research Group on Motor Neuron Diseases·John Ravits
Oct 8, 2005·American Journal of Respiratory Cell and Molecular Biology·Yin ChenReen Wu
Jan 17, 2014·American Journal of Respiratory Cell and Molecular Biology·Lingxiang ZhuYin Chen
Oct 23, 2016·Cellular and Molecular Life Sciences : CMLS·Rafael Sanjuán, Pilar Domingo-Calap
Feb 26, 2003·American Journal of Respiratory Cell and Molecular Biology·James E GernWilliam W Busse
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Juan RegueraMauricio G Mateu
Jul 28, 2010·Journal of Virology·Rafael SanjuánRobert Belshaw
Jul 12, 2005·Antiviral Chemistry & Chemotherapy·Eddy Littler, Bo Oberg
Mar 23, 2017·American Journal of Respiratory Cell and Molecular Biology·Xu ZhouYin Chen
Jan 6, 2018·Nanoscale·Alejandro ValbuenaMauricio G Mateu
Mar 16, 2018·Microbiology and Molecular Biology Reviews : MMBR·Vadim I Agol, Anatoly P Gmyl
Dec 24, 2018·American Journal of Respiratory Cell and Molecular Biology·Lingxiang ZhuYin Chen
Apr 30, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Elmira MohammadiShaghayegh Haghjooy Javanmard
Aug 16, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Philip R. Wyde

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.