Wine polyphenols and ethanol do not significantly scavenge superoxide nor affect endothelial nitric oxide production

The Journal of Nutritional Biochemistry
Albert HuismanErnst E Van Faassen

Abstract

Epidemiological studies have shown that moderate intake of red wine reduces the risk of coronary heart disease. It has been proposed that the antiatherogenic effect be due to the scavenging of reactive oxygen species by polyphenols and ethanol or an effect on endothelial nitric oxide (NO) production. We have determined the reaction rates of superoxide with four different polyphenols and ethanol. The superoxide reaction rates were determined at 37 degrees C and pH 7.4 using competitive spin trapping and electron paramagnetic resonance (EPR) spectroscopy. Ethanol did not scavenge superoxide. For the polyphenols catechin, epicatechin, gallic acid, and quercetin, we find rate constants of respectively 2.3*10(4), 2.2*10(4), 2.3*10(3) and 1.9*10(4)(mole per second)(-1). Polyphenols can only exert a significant scavenging effect, if the plasma concentration reach sufficiently high levels. At concentrations found in vivo (low nanomolar range), the scavenging of superoxide by polyphenols and ethanol is negligible in comparison with endogenous protection against superoxide. Incubation of cultured endothelial cells with 5 micromol/L of catechin, epicatechin, gallic acid, quercetin, or ethanol 0.05% (v/v) did not influence the maximal prod...Continue Reading

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Citations

Feb 16, 2006·Journal of Fluorescence·Ana GomesJosé L F C Lima
Jul 27, 2005·Free Radical Biology & Medicine·Laila H Abou-AgagDale A Parks
Aug 4, 2012·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Ilse C SchrieksHenk F J Hendriks
Jun 6, 2009·Nitric Oxide : Biology and Chemistry·Christoph A Schmitt, Verena M Dirsch
Sep 3, 2013·Asian Pacific Journal of Tropical Biomedicine·Nadjet Debbache-BenaidaDjebbar Atmani
Jul 1, 2016·Acta Veterinaria Scandinavica·Ramona BabosovaMonika Martiniakova
Mar 22, 2008·Journal of Cardiovascular Pharmacology·Siu-Lung ChanJeffrey Atkinson
Jun 6, 2014·Food & Function·J DuarteF Perez-Vizcaino

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