Wipe-test and patch-test for alcohol misuse based on the concentration ratio of fatty acid ethyl esters and squalene CFAEE/CSQ in skin surface lipids

Forensic Science International : Synergy
Fritz PragstC Dyes

Abstract

Fatty acid ethyl esters (FAEE) are known to be formed in blood and almost all human tissues after alcohol consumption and to be incorporated from sebum into hair where they can be used as long-term markers for excessive alcohol consumption. In order to examine whether skin surface lipids which consist mainly of sebum are an equally useful matrix for measurement of FAEE as alcohol abuse markers, samples were collected by a wipe-test from the forehead of 13 teetotallers, 16 social drinkers, 10 death cases with known recent alcohol misuse and five death cases without indications of alcohol misuse. The samples were analysed by headspace solid-phase microextraction and gas chromatography-mass spectrometry for ethyl myristate, ethyl palmitate, ethyl oleate and ethyl stearate and by high performance liquid chromatography with photodiode array detector for squalene, (SQ), as a natural reference substance which the FAEE concentrations were related to. The ratio mFAEE/mSQ ranged between 0.16 and 1.12 ng/microg (mean 0.34 ng/microg) for the teetotallers and between 0.08 and 0.94 ng/microg (mean 0.37 ng/microg) for the social drinkers with no significant difference between both groups. For the alcoholics 2.4-24.2 ng/microg (mean 13.1 ng/mi...Continue Reading

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Citations

May 4, 2007·Analytical and Bioanalytical Chemistry·Fritz Pragst
Nov 7, 2007·Pharmaceutical Research·Christophe HerkenneRichard H Guy
Nov 19, 2010·Bioanalysis·Yiannis C FiamegosConstantine D Stalikas
Jan 27, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zih-Rou HuangJia-You Fang
Jan 12, 2010·Forensic Science International : Synergy·Silke SüsseFritz Pragst
Jul 25, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Aleksandr B Stefaniak, Christopher J Harvey
Apr 21, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fritz Pragst, Marie A Balikova
Mar 1, 2006·The Journal of Laboratory and Clinical Medicine·Clark C KuligGregory T Everson
May 22, 2016·Clinical Biochemistry·Natalie KummerChristophe P Stove
Dec 4, 2019·Journal of Analytical Toxicology·Nadia De Giovanni, Daniela Marchetti

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