Wipe test for the detection of indoor allergens

Allergy
R PolziusJ Mahn

Abstract

The determination of house dust mite and cat allergens is a prerequisite for qualified assessment of exposure risk indoors. We describe the validation of integrated wipe tests comprising a porous sampling pin for the collection of settled house dust and an immunochemical test set-up for specific analysis of dust associated allergens. Wipe tests comprised an immunochromatographic test system and a porous sampling pin for collecting dust by manually wiping a surface. Two different wipe tests were developed with specificity to cat allergen (Fel d 1) and to dust mite allergen (Der p 2/f 2). One-hundred and fifty on-site determinations for each of the allergens in 50 different households completed the field study to validate the analytical system. Reference analysis was based on collection of dust with a vacuum cleaner and analysis of dust associated allergens by ELISA. The wipe tests were found to produce semiquantitative results (class 0-3) on-site within 10 min of analysis time. For dust mite (cat) allergen the median of class 0-3 was 119 (263), 812 (844), 2708 (5670) and 8000 (56 800) ng/g dust, respectively. With respect to cut-off levels for dust mite (cat) allergens the clinical sensitivity was 62% (79%) and the clinical spec...Continue Reading

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Citations

May 7, 2013·Biosensors & Bioelectronics·Ryoji KuritaOsamu Niwa
Mar 26, 2008·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Wendy CozenThomas M Mack
Feb 7, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A TsayUNKNOWN Multi-Centre Study Group
Jun 23, 2009·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Torsten SchäferAnja Krauspe
Feb 25, 2005·Allergy·J K SercombeE R Tovey

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