With a little help from a friend: increasing HIV transduction of monocyte-derived dendritic cells with virion-like particles of SIV(MAC)

Gene Therapy
C GoujonA Cimarelli

Abstract

Modification of dendritic cells (DCs) is a promising avenue for gene therapy purposes, given the versatility and the multiplicity of functions of these cells. In this study, we show that preincubation of monocyte-derived DCs with low amounts of non-infectious virion-like particles derived from the simian immunodeficiency virus (SIV(MAC) VLPs) increases up to 10-fold the efficiency of transduction by HIV-1 lentiviral vectors at low multiplicity of infections yielding up to 90% of transduced cells, in the absence of alterations of DCs behavior. This effect is restricted to DCs and specified by the viral accessory protein Vpx. Thus, preincubation with empty VLPs of SIV(MAC) can be used in transduction protocols to increase the efficacy of HIV-1-mediated modification of DCs.

References

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Feb 28, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Philippe-Emmanuel MangeotJean-Luc Darlix

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Citations

Feb 22, 2012·Nature Immunology·Nan Yan, Zhijian J Chen
Oct 19, 2012·The Journal of Biological Chemistry·Gregory BergerAndrea Cimarelli
Feb 22, 2013·The Journal of Biological Chemistry·Junpeng YanJacek Skowronski
Nov 20, 2009·Journal of Virology·Thomas GrambergNathaniel R Landau
Oct 19, 2012·Journal of Virology·Stéphanie DurandAndrea Cimarelli
Sep 14, 2012·Journal of Virology·Henning HofmannNathaniel R Landau
Dec 21, 2012·Journal of Virology·Stéphanie CordeilAndrea Cimarelli
Feb 28, 2013·Retrovirology·Caroline GoujonMichael H Malim
Mar 19, 2013·Retrovirology·Thomas GrambergNathaniel R Landau
Apr 13, 2010·Retrovirology·Diana AyindeFlorence Margottin-Goguet
Jul 12, 2011·Retrovirology·Corine St Gelais, Li Wu
Nov 9, 2012·Retrovirology·Tao ZhouYong-Hui Zheng
Sep 22, 2009·PloS One·Silke SchüleMatthias Schweizer
Nov 4, 2011·Viruses·Stéphanie Durand, Andrea Cimarelli
Oct 14, 2011·Viruses·Vicente Planelles
Sep 8, 2012·Future Microbiology·Zhangming ChenSongcheng Ying
Nov 20, 2013·Trends in Immunology·Olivier ManchesNina Bhardwaj
Dec 21, 2013·Nature·Gilad DoitshWarner C Greene
Jan 15, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Semih U TareenScott H Robbins
Apr 22, 2014·Nature Immunology·Hannah E Volkman, Daniel B Stetson
May 24, 2014·Cellular and Molecular Life Sciences : CMLS·Jan Rehwinkel

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