Withania somnifera root extract inhibits fatty acid synthesis in prostate cancer cells

Journal of Traditional and Complementary Medicine
Su-Hyeong KimShivendra V Singh

Abstract

Prior research argues for a role of increased de novo fatty acid synthesis in pathogenesis of prostate adenocarcinoma, which remains a leading cause of cancer-associated mortality in American men. A safe and effective inhibitor of fatty acid synthesis is still a clinically unmet need. Herein, we investigated the effect of ethanol extract of Withania somnifera root (WRE) standardized for one of its components (withaferin A) on fatty acid synthesis using LNCaP and 22Rv1 human prostate cancer cells. Withania somnifera is a medicinal plant used in the Ayurvedic medicine practiced in India. Western blotting and confocal microscopy revealed a statistically significant decrease in protein levels of key fatty acid metabolism enzymes including ATP citrate lyase (ACLY), acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FASN), and carnitine palmitoyltransferase 1A (CPT1A) in WRE-treated cells compared with solvent control. The mRNA levels of ACLY, ACC1, FASN, and CPT1A were also lower in WRE-treated cells in comparison with control. Consequently, WRE treatment resulted in a significant decrease in intracellular levels of acetyl-CoA, total free fatty acids, and neutral lipid droplets in both LNCaP and 22Rv1 cells. WRE exhibited greater...Continue Reading

Citations

May 6, 2021·Nutrition and Cancer·Vikrant MehtaAnjana Munshi
Jul 20, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology· Sajida, Ashwini Prabhu

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Methods Mentioned

BETA
transfection
flow cytometry
histone acetylation
transgenic

Software Mentioned

IT
ImageJ
GraphPad Prism
SCAN
UN

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