Within-sample variation of fecal glucocorticoid measurements

General and Comparative Endocrinology
Joshua J Millspaugh, Brian E Washburn

Abstract

Fecal glucocorticoid metabolite analysis is a useful tool for monitoring adrenocortical activity in captive and free-ranging wildlife. Glucocorticoid metabolites may not be evenly distributed within fecal samples and this variability could affect the interpretation of glucocorticoid metabolite measurements. Furthermore, the precision (i.e., repeatability of measurements) of fecal glucocorticoid measurements from well-mixed samples is unknown. We collected fresh white-tailed deer (Odocoileus virginianus) feces at various times pre- and post-adrenocorticotropin injection to provide samples with low (<75 ng/g), medium (75-90 ng/g), and high (>90 ng/g) glucocorticoid concentrations in case variability differs in samples of dissimilar hormone metabolite concentrations. We compared two sampling methods (selection of three pellet groups [one from each end of the fecal mass and one from the center] versus sampling three small portions of the thoroughly mixed fecal mass) to estimate within-sample variation of glucocorticoid metabolites. Glucocorticoid metabolite measures from pellet groups were higher than fecal glucocorticoid measures from mixed samples in the low (F=3.10; df = 1,56; P=0.08) and medium concentration (F=7.28; df = 1,50;...Continue Reading

References

Mar 1, 1993·Journal of Reproduction and Fertility·S K WasserS L Monfort
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Feb 26, 1998·Journal of Wildlife Diseases·R A GarrottJ G Cook
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Sep 13, 2002·General and Comparative Endocrinology·Brian E Washburn, Joshua J Millspaugh

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Citations

Nov 23, 2013·PloS One·Nicole Ashley DaviesAdrian Bradley
Jul 11, 2013·The Journal of Endocrinology·Nicole DaviesAdrian Bradley
Feb 27, 2007·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Jessica M KeayTaranjit Kaur
Jul 14, 2010·American Journal of Primatology·David J PappanoJacinta C Beehner
Mar 3, 2012·PloS One·David S JachowskiJoshua J Millspaugh
Oct 25, 2016·Primates; Journal of Primatology·Taufiq Purna NugrahaTony Weingrill
Sep 15, 2004·General and Comparative Endocrinology·Joshua J Millspaugh, Brian E Washburn
Aug 5, 2017·Ecological Applications : a Publication of the Ecological Society of America·Brett R JesmerMatthew J Kauffman
Feb 9, 2018·Scientific Reports·Beatriz Sánchez-GonzálezRoberto Latorre
Dec 29, 2018·Conservation Physiology·Grace L ParikhJoseph K Bump
Dec 19, 2017·Conservation Physiology·Diana J R LaffertyL Scott Mills
Jan 8, 2021·Die Naturwissenschaften·Loren L FardellEdward J Narayan
Oct 19, 2020·Reproduction in Domestic Animals = Zuchthygiene·Ana PiñeiroIsabel Barja
Jan 12, 2021·Ecological Applications : a Publication of the Ecological Society of America·Brett R JesmerJacob R Goheen
Apr 23, 2019·General and Comparative Endocrinology·Frédéric Dulude-de BroinGabriela F Mastromonaco
Jun 16, 2019·General and Comparative Endocrinology·Yuki TanakaJosé Maurício Barbanti Duarte
Jul 10, 2012·Applied Animal Behaviour Science·Steven J SchapiroPramod N Nehete

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