Apr 5, 2020

Characterization of exosporium layer variability of Clostridioides difficile spores in the epidemically relevant strain R20291.

BioRxiv : the Preprint Server for Biology
Marjorie Pizarro-GuajardoDaniel Paredes-Sabja

Abstract

Clostridioides difficile is a Gram-positive anaerobic intestinal pathogenic bacterium and the causative agent of antibiotic-associated diarrhea and spores are the transmission vehicle of the disease. In C. difficile spores, the outermost exosporium layer is the first barrier of interaction with the host and should carry spore ligands involved in spore-host interactions. C. difficile forms two types of spores (i.e., thin and thick exosporium layers). In this communication, we contribute to understand several biological aspects of these two exosporium morphotypes. By transmission electron microscopy, we demonstrate that both exosporium morphotypes appear simultaneously during sporulation and that the laminations of the spore coat are formed under anaerobic conditions. Nicodednz density-gradient allows enrichment of spores with a thick-exosporium layer morphotype and presence of polar appendage. Using translational fluorescent fusions with exosporium proteins BclA3, CdeA, CdeC and CdeM as well as with several spore coat proteins, we observed that expression intensity and distribution of SNAP-translational fusions in R20291 strain is highly heterogeneous. Electron micrographs demonstrate that multicopy expression of CdeC, but not C...Continue Reading

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Mentioned in this Paper

Computer Software
Patient Portals
Study
Small Nuclear RNA
Genes, Plant
Post-Transcriptional Regulation
Crops, Agricultural
Genes
Cellular Response to Abiotic Stimulus
Transcription, Genetic

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