WNK1 is involved in Nogo66 inhibition of OPC differentiation

Molecular and Cellular Neurosciences
Zhaohuan ZhangXiaohui Xu

Abstract

LINGO-1 is a transmembrane receptor expressed primarily in the central nervous system (CNS) and plays an important role in myelination. Recent studies have indicated that it is also involved in oligodendrocyte precursor cell (OPC) survival and differentiation; however, the downstream signaling pathway underlying OPC development is unknown. In our previous study, we found that LINGO-1 is associated with WNK1 in mediating Nogo-induced neurite extension inhibition by RhoA activation. In an effort to identify the role of LINGO-1-WNK1 in OPCs, we first confirmed that WNK1 is also expressed in OPCs and co-localized with LINGO-1, which suppresses WNK1 expression by RNA interference-attenuated Nogo66-induced inhibition of OPC differentiation. Furthermore, we mapped the WNK1 kinase domain using several fragmented peptides to identify the key region of interaction with LINGO-1. We found that a sequence corresponding to the D6 peptide is necessary for the interaction. Finally, we found that using the TAT-D6 peptide to introduce D6 peptide into primary cultured OPC inhibits the association between LINGO-1 and WNK1 and significantly attenuates Nogo66-induced inhibition of OPC differentiation. Taken together, our results show that WNK1, via ...Continue Reading

References

Apr 1, 1997·Multiple Sclerosis : Clinical and Laboratory Research·R J Franklin, W F Blakemore
Sep 5, 2002·Nature Reviews. Neuroscience·Robin J M Franklin
Feb 18, 2004·Nature Neuroscience·Sha MiR Blake Pepinsky
May 17, 2005·Nature Neuroscience·Sha MiR Blake Pepinsky
Oct 6, 2005·Neuron·Monique Dubois-DalcqRobin J M Franklin
Jan 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mark R KotterRobin J M Franklin
Jan 19, 2006·Journal of Cell Science·Xiaohui XuYizhang Chen
Jan 5, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xinhua LeeSha Mi
Apr 14, 2009·The Journal of Biological Chemistry·Zhaohuan ZhangCheng He
May 19, 2009·Neuroscience Letters·Xiao-Hui XuCheng He
Apr 20, 2011·The Journal of Cell Biology·Céline CaillavaAnne Baron-Van Evercooren
Jun 23, 2011·Annual Review of Neuroscience·Stephen P J FancyDavid H Rowitch
Mar 14, 2013·The Journal of Biological Chemistry·Zhaohuan ZhangCheng He
Apr 18, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Heung Sun KwonStanislav I Tomarev

❮ Previous
Next ❯

Citations

Jun 18, 2017·Neurochemical Research·Zhao-Huan ZhangXiao-Hui Xu
Jan 31, 2017·Cellular and Molecular Neurobiology·Zhao-Huan ZhangXiao-Hui Xu
Oct 19, 2017·Scientific Reports·Chantal Hoi Yin CheungHsueh-Fen Juan
Oct 3, 2017·Neural Regeneration Research·Simon FoaleZubair Ahmed
Apr 26, 2020·Scientific Reports·Zhao-Huan ZhangXiao-Hui Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.