Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells

Biochemical and Biophysical Research Communications
Yukiteru OujiShigeaki Ishizaka

Abstract

Wnt-10b was originally isolated from lymphoid tissue and is known to be involved in a wide range of biological actions, while recently it was found to be expressed early in the development of hair follicles. However, few studies have been conducted concerning the role of Wnt-10b with the differentiation of skin epithelial cells. To evaluate its role in epithelial differentiation, we purified Wnt-10b from the supernatant of a concanavalin A-stimulated lymphocyte culture using an affinity column and investigated its effects on the differentiation of adult mouse-derived primary skin epithelial cells (MPSEC). MPSEC cultured with Wnt-10b showed morphological changes from cuboidal to spindle-shaped with inhibited proliferation, and also obtained characteristics of the hair shaft and inner root sheath of the hair follicle, represented by red-colored Ayoub Shklar staining, and reactions to AE-13 and AE-15 as seen with immunocytology. Further, RT-PCR analysis demonstrated the expression of mRNA for keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5, in Wnt-10b-treated MPSEC. In addition, involvement of the canonical Wnt signal pathway was demonstrated by a TCF re...Continue Reading

References

Jul 1, 1991·Journal of the American Academy of Dermatology·L C Sperling
Aug 11, 2000·Science·S E RossO A MacDougald
Nov 1, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N Barker, H Clevers
Nov 1, 2000·Cell·M Bienz, H Clevers
Nov 13, 2001·Developmental Cell·E FuchsR DasGupta
Mar 29, 2002·The Hematology Journal : the Official Journal of the European Haematology Association·F J Staal, H Clevers
May 2, 2002·Cell·Marc van de WeteringHans Clevers
May 23, 2002·Developmental Cell·Thomas AndlSarah E Millar
Jun 1, 2002·Science·Randall T MoonNorbert Perrimon
Feb 21, 2003·Biochemical and Biophysical Research Communications·Christina N BennettJessica Schwartz
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Silvia MarettoStefano Piccolo
May 21, 2003·Genes & Development·Laura Alonso, Elaine Fuchs
Aug 10, 2004·Journal of Mammary Gland Biology and Neoplasia·Keith R Brennan, Anthony M C Brown
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Christina N BennettOrmond A MacDougald
Jun 18, 2005·Genes & Development·William E LowryElaine Fuchs
Feb 14, 2006·Biochemical and Biophysical Research Communications·Yukiteru OujiShigeaki Ishizaka

❮ Previous
Next ❯

Citations

Jun 27, 2009·Pathology Oncology Research : POR·Ferenc SiposBéla Molnár
Aug 7, 2013·Biochemical and Biophysical Research Communications·Yukiteru OujiMasahide Yoshikawa
Apr 13, 2011·World Journal of Gastroenterology : WJG·Ferenc Sipos, Györgyi Muzes
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ken IwatsukiRobert F Margolskee
Jun 16, 2010·Journal of Bioscience and Bioengineering·Yukiteru OujiShigeaki Ishizaka
Dec 2, 2014·The Journal of Investigative Dermatology·Yukiteru OujiMasahide Yoshikawa
Mar 12, 2011·Clinical and Experimental Dermatology·Y-H LiT Yang
Apr 24, 2014·Arthritis & Rheumatology·Susanne Roser-PageM Neale Weitzmann
May 13, 2006·Biochemical and Biophysical Research Communications·Yukiteru OujiShigeaki Ishizaka
Dec 25, 2007·Biochemical and Biophysical Research Communications·Yukiteru OujiShigeaki Ishizaka
Sep 3, 2009·Cell Metabolism·Masakazu TerauchiRoberto Pacifici
Jun 6, 2007·Biochemical and Biophysical Research Communications·Yukiteru OujiShigeaki Ishizaka
Aug 12, 2017·PloS One·Fraser L CollinsNarayanan Parameswaran
Aug 24, 2012·Experimental Dermatology·Weon Ju LeeDo Won Kim
Jun 2, 2017·Frontiers in Bioengineering and Biotechnology·Cristina SobacchiCiro Menale

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
Yukiteru OujiShigeaki Ishizaka
Biochemical and Biophysical Research Communications
Yukiteru OujiShigeaki Ishizaka
Biochemical and Biophysical Research Communications
Yukiteru OujiShigeaki Ishizaka
© 2021 Meta ULC. All rights reserved