Wnt-3a Induces Epigenetic Remodeling in Human Dental Pulp Stem Cells.

Cells
V Uribe-EtxebarriaNerea Subirán

Abstract

Dental pulp stem cells (DPSCs) from adult teeth show the expression of a very complete repertoire of stem pluripotency core factors and a high plasticity for cell reprogramming. Canonical Wnt and Notch signaling pathways regulate stemness and the expression of pluripotency core factors in DPSCs, and even very short-term (48 h) activations of the Wnt pathway induce a profound remodeling of DPSCs at the physiologic and metabolic levels. In this work, DPSC cultures were exposed to treatments modulating Notch and Wnt signaling, and also induced to differentiate to osteo/adipocytes. DNA methylation, histone acetylation, histone methylation, and core factor expression levels where assessed by mass spectroscopy, Western blot, and qPCR. A short-term activation of Wnt signaling by WNT-3A induced a genomic DNA demethylation, and increased histone acetylation and histone methylation in DPSCs. The efficiency of cell reprogramming methods relies on the ability to surpass the epigenetic barrier, which determines cell lineage specificity. This study brings important information about the regulation of the epigenetic barrier by Wnt signaling in DPSCs, which could contribute to the development of safer and less aggressive reprogramming methodol...Continue Reading

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Citations

Jun 12, 2020·Mechanisms of Ageing and Development·Rafael Sênos DemarcoD Leanne Jones
Apr 4, 2021·International Journal of Molecular Sciences·Jon LuzuriagaGaskon Ibarretxe
May 29, 2021·Stem Cells International·Jingqiu ChenJun Zhang
Aug 28, 2021·International Journal of Molecular Sciences·Zhuo XieZhengmei Lin

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Methods Mentioned

BETA
histone acetylation
acetylation
mechanical dissociation
PCR
flow cytometry

Software Mentioned

Bank
Primer
SPSS Statistics
CFX [UNK]
Kaluza G for Gallios Acquisition
- Blast
Graph Pad
Fiji
ImageJ
Excel

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