DOI: 10.1101/488718Dec 8, 2018Paper

Wnt/β-catenin signaling is required for the development of multiple nephron segments

BioRxiv : the Preprint Server for Biology
Patrick DeaconJoo-Seop Park

Abstract

The nephron is composed of distinct segments that perform unique physiological functions to generate urine. Little is known about how multipotent nephron progenitor cells differentiate into different nephron segments. It is well known that Wnt/β-catenin signaling regulates the maintenance and commitment of mesenchymal nephron progenitors during kidney development. However, it is not fully understood how it regulates nephron patterning after nephron progenitors undergo mesenchymal-to-epithelial transition. To address this, we performed β-catenin loss-of-function and gain-of-function studies in epithelial nephron progenitors in the mouse kidney. Consistent with a previous report, the formation of the renal corpuscle was defective in the absence of β-catenin. Interestingly, we found that epithelial nephron progenitors lacking β-catenin were able to form presumptive proximal tubules but that they failed to further develop into differentiated proximal tubules, suggesting that Wnt/β-catenin signaling plays a critical role in proximal tubule development. We also found that epithelial nephron progenitors lacking β-catenin failed to form the distal tubules. Constitutive activation of Wnt/β-catenin signaling blocked the proper formation ...Continue Reading

Related Concepts

Kidney
Kidney Tubules, Proximal
Laboratory mice
Stem Cells
Urine
Proximal
Renal Corpuscle Structure
Nephron brand of racepinephrine hydrochloride
Wnt Proteins
Mesenchymal Stem Cells

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