Wnt/β-catenin signaling pathway participates in the effect of miR-626 on oral squamous cell carcinoma by targeting RASSF4.

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Sheng-Hai CuiYan Yan

Abstract

The role of miR-626 in oral squamous cell carcinoma (OSCC) was investigated by targeting RASSF4. The miR-626 and RASSF4 expression was detected in normal oral mucosa or OSCC tissues and OSCC or normal cells. The methylation status of RASSF4 was analyzed using methylation-specific polymerase chain reaction (PCR). The cytoplasmic/nuclear ratios (C/N ratios) targeted by miR-626 were examined using microarray, followed by a dual-luciferase reporter assay. The subcellular localization of RASSF4 and miR-626 in OSCC cells was determined using RNA fluorescence in situ hybridization (FISH) and immunocytochemistry (ICC), respectively. Ca9-22 and HSC2 cells were divided into mock, inhibitor NC, miR-626 inhibitor, scramble, RASSF4 and miR-626 mimic + RASSF4 groups, and then CCK-8, Annexin V-FITC/PI, wound healing, Transwell, qRT-PCR and western blotting assays were performed. OSCC tissues and cells had increased miR-626 expression and decreased RASSF4 expression. Patients with RASSF4 methylation had lower RASSF4 expression than those without methylation. In addition, a negative correlation between miR-626 and RASSF4 was found in OSCC tissues, both of which were correlated with the pathological grade, pathological stage, lymph node metastas...Continue Reading

References

Dec 3, 2004·Cancer Research·Kristin EckfeldGeoffrey J Clark
Jun 1, 2007·Molecular Carcinogenesis·Claudia SeidelReinhard Dammann
Aug 1, 2008·Nature·Matthias SelbachNikolaus Rajewsky
May 27, 2014·International Journal of Oncology·Lun DongHengzhu Zhang
Jun 8, 2014·Breast Cancer Research and Treatment·Manoj M PillaiPeter Kabos
Sep 25, 2014·Science Signaling·David M Gonzalez, Damian Medici
Jul 8, 2015·International Journal of Molecular Sciences·Chou-Kit ChouChien-Chih Chiu
Oct 21, 2015·Critical Reviews in Oncology/hematology·Shivangi MishraVibha Rani
Nov 4, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yong HanEnhua Wang
Feb 26, 2016·Molecular Cancer·Silvia Juliana Serrano-GomezSuresh K Alahari
Mar 27, 2016·Genes, Chromosomes & Cancer·Rebecca TowleCathie Garnis
Sep 7, 2016·Scientific Reports·Vanmathy Kasimanickam, John Kastelic
Jun 11, 2017·The Journal of Cell Biology·Yu-Ju ChenJen Liou
Jun 22, 2017·The Journal of Cell Biology·Eamonn J Dickson
Feb 8, 2018·Annual Review of Pathology·Vivek Mittal
May 26, 2018·Briefings in Bioinformatics·Tingting ShaoYongsheng Li
Jun 25, 2018·In Vivo·Yaeko HaraTadayoshi Kaneko
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuan YaoRui-Xin He
Jul 7, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Ahmet ElbayHakan Ozdemir
Sep 8, 2019·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Li-Xia QinChun-Yu Wang
Apr 20, 2020·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Zhao XiangYinghui Tan
May 24, 2020·Oral Oncology·Alhadi AlmangushIlmo Leivo
Sep 21, 2020·Medical Molecular Morphology·Bhoj Raj AdhikariYoshihiro Abiko
Dec 29, 2020·Frontiers in Oncology·Daniel Peña-OyarzúnAlfredo Criollo

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