Wnt/β-catenin signaling regulates Dental Pulp Stem Cells' responses to pulp injury by resinous monomers

Dental Materials : Official Publication of the Academy of Dental Materials
Athina BakopoulouWerner Geurtsen

Abstract

Aim of this study was to investigate whether Dental Pulp Stem Cells-DPSCs responses to pulp injury caused by resinous monomers is be mediated through activation of Wnt/β-catenin signaling. DPSCs cultures were established from third molars of healthy donors and characterized for stem cell markers with flow cytometry. Cells were exposed to TEGDMA (T: 0.5-2mM) with or without presence of the Wnt-1 ligand (W:25-100ng/ml) or the GSK3β inhibitor Lithium (L:1-10mM), used both as activators of Wnt/β-catenin signaling. Cell viability was evaluated by MTT assay, cell cycle profiles by flow cytometry and expression of key molecules of Wnt/β-catenin signaling by Real-time PCR and Western Blot. DPSC exposure to TEGDMA caused a concentration-dependent cytotoxicity, accompanied by G1 arrest at lower and G2/M arrest at higher concentrations or after prolonged exposure. Lithium caused a dual effect, by stimulating/inhibiting cell proliferation at lower/higher concentrations respectively and causing a G2/M arrest in a concentration-dependent manner. Wnt signaling could be activated in DPSCs after Lithium or Wnt-1 treatment, as shown by accumulation of β-catenin, its translocation into the nucleus and enhanced expression of key pathway players, l...Continue Reading

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Citations

Jun 15, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Athina BakopoulouPetros Koidis
Sep 22, 2016·Stem Cell Research & Therapy·Naoki UmemuraHiroshi Sakagami
Nov 8, 2017·Expert Opinion on Biological Therapy·Christian Morsczeck, Torsten E Reichert
May 16, 2019·Stem Cells International·Wenjing XuWeiliang Qiu
Oct 19, 2017·Molecular Medicine Reports·Yali YangLei Zhao
Sep 22, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Qianmin OuXuefeng Lin
Oct 15, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Eirini AthanasiadouAthina Bakopoulou
Jun 28, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·Abeer AlaohaliPaul T Sharpe
Jun 18, 2021·World Journal of Stem Cells·Jing-Yao YinDan-Dan Ma

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