Wnt Receptor Frizzled-4 as a Marker for Isolation of Enteric Neural Progenitors in Human Children

Cells
Peter Helmut NeckelLothar Just

Abstract

Identification and isolation of neural progenitor cells from the human enteric nervous system (ENS) is currently hampered by the lack of reliable, specific markers. Here, we define the Wnt-receptor frizzled-4 as a marker for the isolation of enteric neural progenitor cells derived from paediatric gut samples. We show that the Wnt-receptor frizzled-4 is expressed in the human colon and in Tunica muscularis-derived enterospheres. To obtain a purified culture, we carried out fluorescence-activated cell sorting (FACS) using PE-conjugated frizzled-4 antibodies. Frizzled-4positive cells gave rise to neurosphere-like bodies and ultimately differentiated into neurons as revealed by BrdU-proliferation assays and immunocytochemistry, whereas in frizzled-4negative cultures we did not detect any neuronal and glial cells. By using a patch-clamp approach, we also demonstrated the expression of functional sodium and potassium channels in frizzled-4positive cell cultures after differentiation in vitro.

References

May 17, 2002·Progress in Neurobiology·Pankaj Sah, E S Louise Faber
Jul 23, 2004·Birth Defects Research. Part C, Embryo Today : Reviews·Martín L BaschMarianne Bronner-Fraser
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Catriona Y Logan, Roel Nusse
Apr 15, 2005·Nature·Tannishtha Reya, Hans Clevers
Apr 9, 2008·Cell Research·Roel Nusse
Nov 26, 2008·Gastroenterology Clinics of North America·Natasha Walzer, Ikuo Hirano
Aug 7, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Min-Tsai LiuMichael D Gershon
Jun 4, 2010·Pathology International·Yuka KishabaToshiro Niki
Feb 22, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M GroverUNKNOWN NIDDK Gastroparesis Clinical Research Consortium (GpCRC)
Dec 12, 2012·Nature Reviews. Gastroenterology & Hepatology·Florian ObermayrHeather M Young
Jan 22, 2015·The Journal of Biological Chemistry·Jacomijn P DijksterhuisGunnar Schulte
Dec 24, 2015·Stem Cells International·Katharina NothelferLothar Just
Dec 24, 2015·Stem Cells International·Peter Helmut NeckelLothar Just
Apr 10, 2016·Developmental Biology·Alan J BurnsPieter Vanden Berghe
Oct 23, 2015·Cellular and Molecular Gastroenterology and Hepatology·Benjamin N RolloDonald F Newgreen
Feb 18, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lincon A Stamp

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence-activated cell sorting
FCS
flow cytometry
FACS

Software Mentioned

FlowJo

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.