Wnt signaling pathway correlates with ossification of the spinal ligament: A microRNA array and immunohistochemical study

Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association
Takafumi YayamaShinji Imai

Abstract

Ossification of the posterior longitudinal ligament or the ligamentum flavum parallels endochondral ossification. Cell differentiation at the ossification front is known to be important during this process, although the factors regulating its initiation and progression are still unclear. The purpose of this study was to identify factors important for the regulation of chondrocyte/osteoblast differentiation during spinal ossification. Ligamentum flavum tissue was isolated from 25 patients who underwent decompressive surgery for cervical ossification of the posterior longitudinal ligament. Tissue sections were used for in vitro culture to obtain primary cells through migration methods. To identify microRNAs associated with ossification of the posterior longitudinal ligament, cultured cells were prepared from the ligamentous tissue (n = 4; continuous type) or from control ligamentous samples harvested from patients with cervical spondylosis without spinal ossification, and analyzed using a microRNA array. The ligamentous sections were also examined by immunohistochemistry for the expression of candidate microRNA target genes. The microRNA array identified 177 factors; 12 of which were expressed at significantly different levels in...Continue Reading

Citations

Jul 2, 2019·Molecular Medicine Reports·Daliang KongFei Wang
Jan 3, 2021·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Xiaoqiu YuanYu Chen

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