WNT signalling in the normal human adult testis and in male germ cell neoplasms.

Human Reproduction
Julia C YoungKate L Loveland

Abstract

Is WNT signalling functional in normal and/or neoplastic human male germ cells? Regulated WNT signalling component synthesis in human testes indicates that WNT pathway function changes during normal spermatogenesis and is active in testicular germ cell tumours (TGCTs), and that WNT pathway blockade may restrict seminoma growth and migration. Regulated WNT signalling governs many developmental processes, including those affecting male fertility during early germ cell development at embryonic and adult (spermatogonial) ages in mice. In addition, although many cancers arise from WNT signalling alterations, the functional relevance and WNT pathway components in TGCT, including germ cell neoplasia in situ (GCNIS), are unknown. The cellular distribution of transcripts and proteins in WNT signalling pathways was assessed in fixed human testis sections with normal spermatogenesis, GCNIS and seminoma (2-16 individuals per condition). Short-term (1-7 h) ligand activation and long-term (1-5 days) functional outcomes were examined using the well-characterised seminoma cell line, TCam-2. Pathway inhibition used siRNA or chemical exposures over 5 days to assess survival and migration. The cellular localisation of WNT signalling components wa...Continue Reading

References

Jul 1, 1993·Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology·Y MizunoS Kitazawa
Apr 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Patrick W B DerksenSteven T Pals
Sep 21, 2004·The Journal of Pathology·Friedemann HoneckerLeendert H J Looijenga
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Catriona Y Logan, Roel Nusse
Nov 18, 2004·The Journal of Biological Chemistry·Qin LiMakoto M Taketo
Sep 15, 2005·Cellular and Molecular Life Sciences : CMLS·I GashawE Winterhager
Sep 6, 2007·Molecular Human Reproduction·Isabella GashawElke Winterhager
Dec 1, 2007·Genes, Chromosomes & Cancer·Jeroen de JongLeendert H J Looijenga
Dec 23, 2008·Methods in Molecular Biology·Nick Barker
Dec 23, 2008·Methods in Molecular Biology·Nick Barker, Maaike van den Born
Jan 2, 2009·Cancer Research·Helen B PearsonAlan R Clarke
Jan 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andy J ChienRandall T Moon
Jan 30, 2009·The Journal of Investigative Dermatology·Andy J ChienRandall T Moon
May 8, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Nady GolestanehMartin Dym
Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
Sep 9, 2009·Development·Renée van Amerongen, Roel Nusse
Sep 18, 2009·Nature·Shih-Min A HuangFeng Cong
Jan 23, 2010·Pathology, Research and Practice·Tomislav VladusićJasna Franekić Colić
Jun 9, 2010·The Journal of Pathology·Riccardo Fodde, Ian Tomlinson
Jun 23, 2011·Journal of Cell Science·Jonathan R YehMakoto C Nagano
Nov 30, 2011·PloS One·Yao-Fu ChangManjeet K Rao
May 24, 2012·The EMBO Journal·Paul Polakis
May 24, 2012·The EMBO Journal·Tomas ValentaKonrad Basler
Jun 12, 2012·Cell·Hans Clevers, Roel Nusse
Nov 22, 2013·Biology of Reproduction·Genevieve E KerrKate L Loveland
Jan 21, 2014·Current Topics in Developmental Biology·Yaser AtlasiRiccardo Fodde
Apr 30, 2015·Gene·Wei-Lai DongWan-Xi Yang
Jun 7, 2015·Journal of Hematology & Oncology·Rohit MathurFelipe Samaniego
Mar 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hinako M Takase, Roeland Nusse
Sep 8, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Dora FabijanovicSemir Vranic
Sep 10, 2016·Human Reproduction·Britta KleinHans-Christian Schuppe
Sep 13, 2016·Oncogene·T ZhanM Boutros
Sep 21, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Aditya BagrodiaDarren R Feldman
May 1, 2017·Developmental Biology·Anne-Amandine ChassotMarie-Christine Chaboissier
Jun 27, 2017·F1000Research·Aravinda-Bharathi Ramakrishnan, Ken M Cadigan

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Citations

Jan 5, 2021·Molecular Human Reproduction·Ruizhi XuePeng Huang
Feb 6, 2021·Molecular Human Reproduction·Ruizhi XuePeng Huang
May 1, 2021·International Journal of Molecular Sciences·Silvia SchmidtovaMichal Chovanec

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