Wnt traffic from endoplasmic reticulum to filopodia

PloS One
Naushad MotiDavid M Virshup

Abstract

Wnts are a family of secreted palmitoleated glycoproteins that play key roles in cell to cell communication during development and regulate stem cell compartments in adults. Wnt receptors, downstream signaling cascades and target pathways have been extensively studied while less is known about how Wnts are secreted and move from producing cells to receiving cells. We used the synchronization system called Retention Using Selective Hook (RUSH) to study Wnt trafficking from endoplasmic reticulum to Golgi and then to plasma membrane and filopodia in real time. Inhibition of porcupine (PORCN) or knockout of Wntless (WLS) blocked Wnt exit from the ER. Wnt-containing vesicles paused at sub-cortical regions of the plasma membrane before exiting the cell. Wnt-containing vesicles were associated with filopodia extending to adjacent cells. These data visualize and confirm the role of WLS and PORCN in ER exit of Wnts and support the role of filopodia in Wnt signaling.

References

Jun 24, 2000·European Journal of Biochemistry·K TanakaT Kadowaki
Jun 14, 2002·Current Biology : CB·Sven PfeifferJean-Paul Vincent
Jan 14, 2005·Development·Xavier Franch-MarroJean-Paul Vincent
May 9, 2006·Cell·Kerstin BartschererMichael Boutros
Dec 5, 2006·Developmental Cell·Ritsuko TakadaShinji Takada
Apr 3, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hideyuki KomekadoAkira Kikuchi
Sep 10, 2010·Journal of Cell Science·Gary S CoombsDavid M Virshup
Nov 5, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jost LeemhuisHans H Bock
Nov 24, 2011·Developmental Biology·Patrick Herr, Konrad Basler
Dec 8, 2011·EMBO Molecular Medicine·Iris AugustinMichael Boutros
Mar 13, 2012·Nature Methods·Gaelle BoncompainFranck Perez
Mar 23, 2012·The Journal of Biological Chemistry·Kate KolesVivian Budnik
Jun 12, 2012·Cell·Hans Clevers, Roel Nusse
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jul 13, 2012·Differentiation; Research in Biological Diversity·Rani NajdiMarian L Waterman
Sep 18, 2012·Nature Cell Biology·Julia Christina GrossMichael Boutros
Dec 22, 2012·Nature Reviews. Cancer·Jamie N Anastas, Randall T Moon
Oct 25, 2013·Journal of Cellular and Molecular Medicine·Maria-Giuliana VannucchiMaria-Simonetta Faussone-Pellegrini
Apr 29, 2014·Developmental Cell·Jia YuDavid M Virshup
May 14, 2014·Development·Zahra KabiriDavid M Virshup
Jul 31, 2014·Nature Methods·Neville E SanjanaFeng Zhang
Sep 12, 2014·Bioscience Reports·Jia Yu, David M Virshup
Jan 6, 2015·Nature Communications·Eliana StanganelloSteffen Scholpp
Jul 18, 2015·Current Opinion in Cell Biology·Guillaume JacquemetJohanna Ivaska
Jan 30, 2016·Journal of Cell Science·Eliana Stanganello, Steffen Scholpp
Aug 30, 2016·Seminars in Cell & Developmental Biology·Dragos CretoiuSanda Maria Cretoiu
Mar 1, 2017·Current Opinion in Genetics & Development·Thomas B Kornberg
Aug 3, 2017·The Journal of Cell Biology·Guillaume JacquemetJohanna Ivaska
Feb 10, 2018·Cell·Jose B Cibelli, John B Gurdon
Mar 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·Gediminas GreiciusDavid M Virshup
May 4, 2018·Nature·Michal Shoshkes-CarmelKlaus H Kaestner
Sep 27, 2018·Histochemistry and Cell Biology·Benjamin Mattes, Steffen Scholpp

❮ Previous
Next ❯

Citations

Nov 2, 2019·Angiogenesis·Daniela UnterleuthnerHelmut Dolznig
Aug 14, 2020·Molecular & Cellular Proteomics : MCP·Molly M HanniganChristopher V Nicchitta
Dec 29, 2020·Cell·Rie NygaardFilippo Mancia
Dec 24, 2019·Developmental Cell·José Ignacio Valenzuela, Franck Perez
Jul 13, 2021·Drug Discovery Today·Avradip ChatterjeeManash K Paul

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence
PCR
transfection
glycosylation
flow cytometry
acylation

Software Mentioned

ImageJ
NIS
GraphPad Prism
FlowJo
Fiji
Imaris
Element Advanced Research
RUSH

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved