Wnt1 Participates in Inflammation Induced by Lipopolysaccharide Through Upregulating Scavenger Receptor A and NF-kB

Inflammation
Wenting ZhaoLiangrong Zheng

Abstract

The study investigated the role of wnt1 in the inflammatory response initiated by lipolysaccharide (LPS), and analyzed the association between wnt1, NF-KB, and inflammatory factors. THP-1 cells were activated with phorbol-12-myristate-13-acetate (PMA) and treated with LPS to induce inflammation. THP-1 cells were transfected with wnt1siRNA and overexpression plasmid to explore the relationship among wnt1, SRA, and NF-KB. Inhibitor of β-catenin and siRNA of FZD1were used to investigate the signaling events involved in SRA activation induced by wnt1. Levels of NF-kB protein and inflammatory cytokines were assessed followingwnt1 siRNA and LPS treatment. PMA activation and LPS treatment of THP-1 cells increased wnt1 protein levels. Wnt1 promoted SRA expression through activation of canonical wnt pathway. Wnt1 increased NF-kB protein levels and enhanced the secretion of IL-6, TNF-α, and iNOS through binding to SRA. These findings suggest that wnt1 increased SRA and NF-kB protein levels and participated in the inflammatory response.

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Citations

Oct 8, 2016·Frontiers in Immunology·Bin Ma, Michael O Hottiger
Jan 13, 2018·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Chao HanWanyu Shi
Jul 5, 2018·Parasite Immunology·Lian Xun PiaoXue Jun Jin
Oct 19, 2017·International Journal of Molecular Medicine·Qing-Tao MengZhong-Yuan Xia
Nov 30, 2019·Frontiers in Immunology·Johanna K LjungbergAntje Blumenthal
Feb 20, 2018·Experimental and Therapeutic Medicine·Ning HuangfuShenghuang Wang
Jul 10, 2017·Journal of Molecular Neuroscience : MN·Alexandre ValléeJean-Noël Vallée

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Methods Mentioned

BETA
ELISA
Transfection
PMA
electrophoresis
Confocal microscopy
optical microscopy

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