WNT2 regulates DNA synthesis in mouse granulosa cells through beta-catenin.

Biology of Reproduction
Hong-Xing WangGerald M Kidder

Abstract

WNTs are secreted extracellular signaling molecules that transduce their signals by binding to G protein-coupled receptors of the frizzled (FZD) family. They control diverse developmental processes, such as cell fate specification, cell proliferation, cell differentiation, and apoptosis. Although WNT signaling has been shown to be essential for development of the ovary, its mechanistic role in folliculogenesis within the adult ovary has not been studied extensively. Therefore, the objective of this study was to investigate the regulation and function of WNT2 signaling in mouse granulosa cells. Immunostaining identified WNT2 as being expressed in granulosa cells throughout folliculogenesis, but with varying signal strength: in sequential sections, WNT2 immunoreactivity was strongest in healthy antral follicles but weak in atretic follicles. Knockdown of WNT2 expression using transfected short interfering RNA decreased DNA synthesis in granulosa cells, whereas WNT2 overexpression using a recombinant viral vector enhanced it. WNT2 knockdown led to accumulation of glycogen synthase kinase-3beta (GSK3B) in the cytoplasm but reduced the expression of beta-catenin. Conversely, WNT2 overexpression reduced the expression of GSK3B in the...Continue Reading

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Citations

Jul 31, 2013·Journal of Assisted Reproduction and Genetics·Edo DzaficIrma Virant-Klun
Sep 8, 2012·Biology of Reproduction·Evelyne LapointeDerek Boerboom
Nov 7, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·M EndoH Iwata
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Dec 17, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Atefeh AbediniDerek Boerboom
Jan 17, 2019·Journal of Biological Engineering·Andrea S K Jones, Ariella Shikanov
Jul 28, 2020·Animal Reproduction·Filiz Tepekoy, Gokhan Akkoyunlu
Oct 21, 2019·Histochemistry and Cell Biology·Filiz TepekoyGokhan Akkoyunlu
Jul 12, 2013·Biology of Reproduction·Hong-Xing WangGerald M Kidder
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Dec 29, 2020·Molecular and Cellular Endocrinology·Luyi ChenFeixue Li
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Apr 29, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chenfeng YuanXu Zhou

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Methods Mentioned

BETA
Transfection
coimmunoprecipitation

Software Mentioned

Adobe Photoshop
Quantity One
Zeiss LSM
Statistical Package for Social Science ( SPSS
SPSS

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