Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2.

Journal of Cell Science
Rama Kamesh Bikkavilli, Craig C Malbon

Abstract

Wnt signaling is initiated upon binding of Wnt proteins to Frizzled proteins and their co-receptors LRP5 and 6. The signal is then propagated to several downstream effectors, mediated by the phosphoprotein scaffold, dishevelled. We report a novel role for arginine methylation in regulating Wnt3a-stimulated LRP6 phosphorylation. G3BP2, a dishevelled-associated protein, is methylated in response to Wnt3a. The Wnt3a-induced LRP6 phosphorylation is attenuated by G3BP2 knockdown, chemical inhibition of methyl transferase activity or expression of methylation-deficient mutants of G3BP2. Arginine methylation of G3BP2 appears to be a Wnt3a-sensitive 'switch' regulating LRP6 phosphorylation and canonical Wnt-β-catenin signaling.

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Citations

Sep 28, 2015·Biochemical and Biophysical Research Communications·Ole Kristensen
Nov 15, 2012·Scientific Reports·Rama Kamesh BikkavilliRobert A Winn
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Dec 23, 2020·Journal of Neurochemistry·Hadjara SidibéChristine Vande Velde
Feb 24, 2021·Development, Growth & Differentiation·Gabriele Colozza, Bon-Kyoung Koo

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Methods Mentioned

BETA
pull-downs
GTPase
pull downs
PCR
pull-down
transfection
transfections
co-immunoprecipitation
electrophoresis

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