Wnt5a contributes to dectin-1 and LOX-1 induced host inflammatory response signature in Aspergillus fumigatus keratitis

Cellular Signalling
Chengye CheGui-Qiu Zhao

Abstract

Fungal keratitis causes devastating corneal ulcers which can result in significant visual impairment and even blindness. As a ligand that activates the non-canonical Wnt signaling pathways, Wnt5a triggers the production of important inflammatory chemokines and the chemotactic migration of neutrophils. In this study we aimed to characterize the role of Wnt5a production, in situ, in vivo and in vitro in response to fungal keratitis. Wnt5a expression in corneas of Aspergillus fumigatus (A. fumigatus) keratitis patients was determined by quantitative polymerase chain reaction (qRT-PCR) and immunofluorescence. In vivo and in vitro experiments were then performed in mouse models and THP-1 macrophages cell cultures infected with A. fumigatus, respectively. C57BL/6 mice were pretreated with siRNAs or neutralizing antibodies for dectin-1, LOX-1 and Wnt5a, or inhibitors of erk1/2 and JNK. Changes in Wnt5a expression were assessed by clinical evaluation, qRT-PCR, immunofluorescence, western blot and bioluminescence imaging system image acquisition. We confirmed that corneal Wnt5a expression increased with A. fumigatus keratitis in patients and a murine model. Wnt5a production was dependent on dectin-1 and LOX-1 expression with contributio...Continue Reading

Citations

Nov 30, 2019·Frontiers in Immunology·Johanna K LjungbergAntje Blumenthal
Jun 17, 2020·Investigative Ophthalmology & Visual Science·Qing ZhangKan He
Nov 19, 2019·Frontiers in Immunology·Suborno JatiMalini Sen
Oct 6, 2020·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Anmar KensaraRadi Masri
Jul 16, 2021·Current Eye Research·Cui LiGuiqiu Zhao
Jan 25, 2022·Experimental and Therapeutic Medicine·Qian ZhangJie Liu

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