Wnt5a Contributes to the Differentiation of Human Embryonic Stem Cells into Lentoid Bodies Through the Noncanonical Wnt/JNK Signaling Pathway

Investigative Ophthalmology & Visual Science
Chenlu HanLixia Luo

Abstract

Recent work has indicated that Wnt5a has a critical role in embryonic development. We investigate whether the Wnt5a-activated noncanonical Wnt pathway is capable of promoting embryonic lens differentiation. A "three-stage" protocol was used to induce lens differentiation of human embryonic stem cells (hESCs) in vitro, and Wnt5a levels were modified by addition of exogenous protein and RNA interference. SP600125 was adopted to inhibit JNK cascades. The number and size of lentoid bodies obtained were measured, and quantitative RT-PCR, Western blotting, and immunofluorescence were used to detect gene and protein expression. The quantity and size of lentoid bodies generated were significantly increased by addition of exogenous Wnt5a. Moreover, expression of lens-specific genes, including CRYAA, CRYAB, BFSP1, and MIP, and the lens fiber differentiation regulator PROX1 were prominently increased. We also observed activation of noncanonical Wnt signaling via upregulation of Dvl2, Rac1, and JNK. When Wnt5a-knockdown hESCs were induced to differentiate, fewer and smaller lentoid bodies resulted. In addition, expression of genes specific to lens was decreased and noncanonical Wnt/JNK pathway activity was downregulated. Accordingly, inhib...Continue Reading

References

Oct 1, 1996·Current Biology : CB·J Yang-SnyderR T Moon
Apr 1, 1997·Trends in Genetics : TIG·R T MoonM Torres
Mar 18, 1999·Nature Genetics·J T WigleG Oliver
Jan 25, 2002·Genome Biology·Jeffrey R Miller
Feb 19, 2002·Mechanisms of Development·Melinda K DuncanStanislav I Tomarev
Feb 19, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Vasantha RaoSamuel Zigler
May 27, 2003·Investigative Ophthalmology & Visual Science·Sotaro OotoMasayo Takahashi
Jun 19, 2003·Developmental Biology·Richard J W StumpJohn W McAvoy
Jun 20, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hong LiuValerie A Wallace
Sep 12, 2003·Developmental Cell·Michael T VeemanRandall T Moon
Jan 27, 2004·Current Opinion in Ophthalmology·Peter J Francis, Anthony T Moore
Apr 1, 2004·Gene Expression Patterns : GEP·S J AngJ W McAvoy
Apr 20, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Rupalatha MaddalaVasantha P Rao
Jul 28, 2004·Investigative Ophthalmology & Visual Science·Jongkyu ChoiChoun-Ki Joo
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Catriona Y Logan, Roel Nusse
Oct 16, 2004·Journal of Cellular Biochemistry·Genevieve M BolandRocky S Tuan
Nov 24, 2004·The International Journal of Developmental Biology·Yongjuan ChenJohn W McAvoy
Aug 16, 2005·Cell Calcium·Aimee D Kohn, Randall T Moon
Mar 28, 2006·Current Topics in Developmental Biology·Craig C Malbon, Hsien-Yu Wang
Jul 15, 2006·Seminars in Cell & Developmental Biology·Courtney KarnerThomas J Carroll
Jan 11, 2007·Seminars in Cell & Developmental Biology·Y ChenJ W McAvoy
Jan 18, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Chonnettia Jones, Ping Chen
Oct 2, 2007·Progress in Retinal and Eye Research·Ales Cvekl, Melinda K Duncan
Jan 6, 2009·Organogenesis·Sabine Fuhrmann
Apr 1, 2009·The Journal of Biological Chemistry·Ginny G FaríasNibaldo C Inestrosa
Sep 9, 2009·Development·Renée van Amerongen, Roel Nusse
Dec 23, 2009·Genesis : the Journal of Genetics and Development·Ondrej MachonZbynek Kozmik
Apr 23, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chunbo YangAles Cvekl
May 12, 2010·Molecular and Cellular Biology·Michiru NishitaYasuhiro Minami
Jun 4, 2010·International Endodontic Journal·L PengX D Zhou
Jan 20, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Minkui LinYiping Chen
Jan 6, 2012·Methods in Molecular Biology·Yuki Sugiyama, John W McAvoy
Nov 8, 2012·Development·Giovanna M ColluKeith Brennan

❮ Previous
Next ❯

Citations

Jul 26, 2019·Investigative Ophthalmology & Visual Science·Vinay Swamy, David McGaughey
Feb 20, 2020·International Journal of Molecular Sciences·Li-Hong WangXiao-Jing Wang
Nov 22, 2020·The Ocular Surface·Navid ManafiAlireza Mashaghi
Oct 12, 2021·Frontiers in Cell and Developmental Biology·Jinyan LiYizhi Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.