Wnt5a/FZD4 Mediates the Mechanical Stretch-Induced Osteogenic Differentiation of Bone Mesenchymal Stem Cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Qingguo GuJie Zhao

Abstract

Mechanical stimulation and WNT signalling have essential roles in regulating the osteogenic differentiation of bone marrow stromal cells (BMSCs) and bone formation. However, little is known regarding the regulation of WNT signalling molecule expression and therefore the osteogenic differentiation of BMSCs during osteogenesis. Microarrays of BMSCs from elderly individuals or patients with osteoporosis (GSE35959) from the GEO database were analysed using GeneSight-Lite 4.1.6 (BioDiscovery) and C2 curated gene sets downloaded from Molecular Signatures Database (MSigDB). Realtime PCR and western blotting were used to measure the expression of the indicated genes. ALP and Alizarin red staining were used to evaluate the osteogenesis of BMSCs. In this study, we investigated whether mechanical loading directly regulates the expression of WNT signalling molecules and examined the role of WNT signalling in mechanical loading-triggered osteogenic differentiation and bone formation. We first studied the microarrays of samples from patients with osteoporosis and found downregulation of the GPCR ligand binding gene set in the BMSCs of patients with osteoporosis. Then, we demonstrated that mechanical stimuli can regulate osteogenesis and bone...Continue Reading

Citations

Jul 28, 2020·Calcified Tissue International·Valeria CarinaGianluca Giavaresi
Mar 3, 2021·Archives of Oral Biology·Jeeranan ManokawinchokeHiroshi Egusa
May 4, 2021·Journal of Cellular and Molecular Medicine·Fatemeh KhodabandehlooMohamadreza Baghaban Eslaminejad
Oct 16, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Lan ZhangHai Tang
Jun 6, 2021·BMC Genomics·Sébastien RiquierThérèse Commes
May 22, 2021·Frontiers in Cell and Developmental Biology·Sarocha SuthonSusan A Krum
Jul 24, 2021·ACS Applied Materials & Interfaces·Sevda Pouraghaei SevariAlireza Moshaverinia
Nov 10, 2021·Tissue Engineering. Part C, Methods·David BecerraHarald C Ott

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