Wnt7a promotes wound healing by regulation of angiogenesis and inflammation: Issues on diabetes and obesity

Journal of Dermatological Science
Wei WangQian Tan

Abstract

Diabetic skin heals wounds poorly. Though obesity is the common risk factor of diabetes mellitus, few studies have investigated its effects on wound healing. This study aimed to evaluate the morphology and possible mechanism of human umbilical vein endothelial cells (HUVEC-C) in response to different levels of glucose and palmitic acid, and explore the role of Wnt7a in wound healing. The functional changes of HUVEC-C and mRNA expression of Wnt signaling were determined by analyzing cell viability, migration, tube formation and rt-PCR in gradients of glucose and palmitic acid. Recombinant Wnt7a protein was injected around wounds made on streptozotocin (STZ) -induced diabetic rats with (HF) or without (DM) high-fat diet. Angiogenesis and inflammatory statement were mainly analyzed by immunohistochemistry, ELISA, cytometry and Western blotting. The expression of Wnt7a significantly decreased in high Glc/PA cultured cells or DM and HF wounded rats. Impaired wound healing was also observed in DM and HF groups. The healing rate significantly accelerated after localized injection with Wnt7a at d10. Moreover, the expression of CD31, eNOS phosphorylation and NO were increased; the reduction of local neutrophils influx, ICAM-1 and IL-6/8...Continue Reading

Citations

Sep 27, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Mengdie LiYijie Shi
Mar 12, 2021·Stem Cell Research & Therapy·Yi LiYayi Hou
May 28, 2021·Journal of Cellular and Molecular Medicine·Xiaobo NieWei-Dong Chen

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