Wogonin induces G1 phase arrest through inhibiting Cdk4 and cyclin D1 concomitant with an elevation in p21Cip1 in human cervical carcinoma HeLa cells

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Li YangQing-Long Guo

Abstract

Wogonin, a naturally occurring flavonoid, has been shown to have tumor therapeutic potential both in vitro and in vivo. To better understand its anticancer mechanism, we examined the effect of wogonin on human cervical carcinoma HeLa cells. In this study, we observed that G1 phase arrest was involved in wogonin-induced growth inhibition in HeLa cells. Over a 24 h exposure of HeLa cells to 90 micromol x L(-1) wogonin, the promoters of G1-S transition, including cyclin D1/Cdk4 and pRb, decreased within 12 h and E2F-1 depleted in the nucleus at the same time. As the G1 phase arrest developed, p53 and the Cdk inhibitor p21Cip1 elevated both at protein and mRNA levels. Furthermore, the up-regulation of p21Cip1 induced by wogonin was dramatically inhibited by siRNA-mediated p53 gene silencing. Collectively, our data suggested that wogonin induced G1 phase arrest in HeLa cells by modulating several key G1 regulatory proteins, such as Cdk4 and cyclin D1, as well as up-regulation of a p53-mediated p21Cip1 expression. This mechanism of wogonin may play an important role in the killing of cancerous cells and offer a potential mechanism for its anticancer action in vivo.

References

Dec 6, 1996·Science·C J Sherr
Jun 13, 1998·Molecular Pathology : MP·R Donnellan, R Chetty
Jul 1, 1999·Genes & Development·C J Sherr, J M Roberts
Dec 22, 1999·The Journal of Clinical Investigation·G I Shapiro, J W Harper
Jun 30, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·W H KimK H Choi
Feb 24, 2001·Stem Cells·E D Israels, L G Israels
Sep 10, 2003·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Suxing LiuMing Liu
Mar 24, 2004·Journal of Ethnopharmacology·Maki SonodaMasataka Moriyasu
Jun 3, 2004·The Journal of Nutrition·Peter J FergusonD James Koropatnick
Nov 19, 2004·Nature·Joan Massagué
May 18, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael BonhamPeter S Nelson
Jul 12, 2005·CNS Drug Reviews·Man Chun TaiHong Xue
Jun 7, 2006·Biological & Pharmaceutical Bulletin·Wei WangXiao-Tang Wang
Sep 19, 2006·International Immunopharmacology·Chiu-Mei LinJen-Hwey Chiu
Feb 16, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Jian Qing YuGuo Lin Zou
Jun 26, 2008·European Journal of Pharmacology·Hai-Wei ZhangQing-Long Guo

❮ Previous
Next ❯

Citations

Aug 28, 2010·Cancer Metastasis Reviews·Limin ShuAh-Ng Kong
Jul 23, 2011·Chinese Medicine·Wen TanYitao Wang
Apr 26, 2012·Scanning·Bingfang Wang, Xuewu Zhang
Oct 24, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Katrin Sak
Jul 15, 2015·Apoptosis : an International Journal on Programmed Cell Death·Yue ZhaoNa Lu
Feb 3, 2015·The American Journal of Chinese Medicine·Byul-Bora ChoiGyoo-Cheon Kim
Nov 8, 2014·Journal of Medicinal Food·Sahng-Wook HahmYong-Suk Son
Dec 6, 2011·Leukemia Research·Allison L ZwingenbergerMichael S Kent
Jan 12, 2020·Molecular Biology Reports·Feng TangBing Zhao
Nov 29, 2017·BioMed Research International·Jiang RuibinGu Linhui
Jun 11, 2014·Oncotarget·Bhaskar KahaliDavid Reisman
Jan 9, 2013·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Min XuQinglong Guo
Feb 2, 2022·Phytotherapy Research : PTR·Kishore BanikAjaikumar B Kunnumakkara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.