DOI: 10.1101/505073Dec 29, 2018Paper

Wolbachia induce cytoplasmic incompatibility and affect mate preference in Habrobracon hebetor to increase the chance of its transmission to the next generation

BioRxiv : the Preprint Server for Biology
Zeynab BagheriMohammad Mehrabadi

Abstract

Wolbachia are common intracellular bacteria that are generally found in arthropods including a high proportion of insects and also some nematodes. This intracellular symbiont can affect sex ratio with a variety of reproductive anomalies in the host, including cytoplasmic incompatibility (CI) in haplodiploides. In this study, we questioned if the parasitoid wasp, Habrobracon hebetor (Hym.: Braconidae), which is one of the most important biological control agents of many lepidopteran larvae, is infected with Wolbachia. To test this, DNA was extracted from adult insects and subjected to PCR using specific primers to Wolbachia target genes. The results showed high rate of Wolbachia infection in this parasitoid wasp. To find out the biological function of Wolbachia in H. hebetor, we removed this bacterium from the wasps using antibiotic treatment (cured wasps). Results of the crossing experiments revealed that Wolbachia induced CI in H. hebetor in which cured females crossed with infected males produced only males, while in the progeny of other crosses, both males and females were observed. Also, our result showed that the presence of Wolbachia in the females increased fecundity and female offspring of this parasitoid wasp. However,...Continue Reading

Related Concepts

Arthropods
Cytoplasm
DNA
Genes
Insecta
Larva
Polymerase Chain Reaction
Wasps
Intracellular
Oligonucleotide Primers

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