Oct 17, 2019

Wolbachia-induced expression of kenny gene in testes affects male fertility in Drosophila melanogaster

Insect Science
John C BiwotYu-Feng Wang

Abstract

Wolbachia are Gram-negative endosymbionts that are known to cause embryonic lethality when infected male insects mate with uninfected females or with females carrying a different strain of Wolbachia, a situation characterized as cytoplasmic incompatibility (CI). However, the mechanism of CI is not yet fully understood, although recent studies on Drosophila melanogaster have achieved great progress. Here, we found that Wolbachia infection caused changes in the expressions of several immunity-related genes, including significant upregulation of kenny (key), in the testes of D. melanogaster. Overexpression of key in fly testes led to a significant decrease in egg hatch rates when these flies mate with wild-type females. Wolbachia-infected females could rescue this embryonic lethality. Furthermore, in key overexpressing testes terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling signal was significantly stronger than in the control testes, and the level of reactive oxygen species was significantly increased. Overexpression of key also resulted in alterations of some other immunity-related gene expressions, including the downregulation of Zn72D. Knockdown of Zn72D in fly testes also led to a significant decre...Continue Reading

  • References62
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Citations

Mentioned in this Paper

Fertility
Embryo
Study
Immune Response
Zn72d protein, Drosophila
Gene Knockdown Techniques
Genes
Drosophila
Oxidative Stress
Gram-Negative Bacteria

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