Abstract
Endosymbiotic Wolbachia bacteria are abundant in the filarial nematodes that cause onchocerciasis (river blindness), including the larvae (microfilariae) that migrate into the cornea. Using a mouse model of ocular onchocerciasis, we recently demonstrated that it is these endosymbiotic bacteria rather than the nematodes per se that induce neutrophil infiltration to the corneal stroma and loss of corneal clarity (Saint Andre et al., Science 295:1892-1895, 2002). To better understand the role of Wolbachia organisms in the pathogenesis of this disease, we examined the fate of these bacteria in the cornea by immunoelectron microscopy. Microfilariae harboring Wolbachia organisms were injected into mouse corneas, and bacteria were detected with antibody to Wolbachia surface protein. Within 18 h of injection, neutrophils completely surrounded the nematodes and were in close proximity to Wolbachia organisms. Wolbachia surface protein labeling was also prominent in neutrophil phagosomes, indicating neutrophil ingestion of Wolbachia organisms. Furthermore, the presence of numerous electron-dense granules around the phagosomes indicated that neutrophils were activated. To determine if Wolbachia organisms directly activate neutrophils, peri...Continue Reading
References
Jul 1, 1977·The American Journal of Tropical Medicine and Hygiene·W J Kozek, H F Marroquin
Dec 1, 1970·Human Pathology·E V Paul, L E Zimmerman
Oct 1, 1996·Parasitology·E J Gutiérrez-PeñaD W Büttner
Jan 1, 1997·Chemical Immunology·E Pearlman
Nov 3, 1998·Parasite Immunology·R K MehlotraE Pearlman
Jan 12, 1999·The Journal of Clinical Investigation·A HoeraufB Fleischer
Feb 17, 1999·Experimental Eye Research·M DaheshiaB T Rouse
Apr 19, 2000·Lancet·A HoeraufD W Büttner
Apr 19, 2000·The Journal of Experimental Medicine·M J TaylorK Bilo
May 16, 2000·Tropical Medicine & International Health : TM & IH·A HoeraufB Fleischer
Jun 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·V Witko-SarsatL Halbwachs-Mecarelli
Sep 29, 2000·Microbes and Infection·N W BrattigF W Tischendorf
Dec 21, 2000·Parasite Immunology·E Pearlman, L R Hall
Feb 24, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·M J Taylor
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L R HallE Pearlman
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J T KaifiE Pearlman
May 30, 2001·Microbes and Infection·N W BrattigA Hoerauf
Mar 9, 2002·Science·Amélie v Saint AndréEric Pearlman
Aug 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Meredith S GregoryBruce R Ksander
Jan 25, 2003·BMJ : British Medical Journal·Achim HoeraufEric Pearlman
Mar 13, 2003·Parasitology Research·L H KramerM Casiraghi
Jun 21, 2003·Filaria Journal·Helen F McGarryMark J Taylor
Sep 2, 2003·Chemical Immunology and Allergy·Eric Y DenkersSoumaya Bennouna
Nov 5, 2003·Veterinary Parasitology·C BazzocchiC Bandi
Aug 4, 2004·Molecular and Biochemical Parasitology·Helen F McGarryMark J Taylor
Citations
Jan 27, 2007·Current Infectious Disease Reports·Kelly L Johnston, Mark J Taylor
May 22, 2009·The Journal of Biological Chemistry·Joseph D TurnerMark J Taylor
Jul 8, 2011·Clinical Microbiology Reviews·Francesca TamarozziMark J Taylor
Sep 19, 2007·Infection and Immunity·Illona Gillette-FergusonEric Pearlman
Mar 23, 2006·Infection and Immunity·Kristina H PorthouseThomas R Klei
Mar 23, 2006·Infection and Immunity·Illona Gillette-FergusonEric Pearlman
Mar 28, 2006·Filaria Journal·Simon TownsonMark J Taylor
Mar 29, 2008·Journal of Leukocyte Biology·Yan SunEric Pearlman
Aug 7, 2010·PLoS Neglected Tropical Diseases·Frédéric LandmannWilliam Sullivan
Jul 14, 2006·Emerging Infectious Diseases·Siobhán M O'ConnorJames M Hughes
Nov 18, 2006·Journal of Leukocyte Biology·Michelle LinEric Pearlman
Aug 27, 2010·Lancet·Mark J TaylorMoses Bockarie
Oct 8, 2014·Parasites & Vectors·Denis VoroninMark J Taylor
Sep 2, 2009·Proteomics·Anil DangiShailja Misra-Bhattacharya
Dec 26, 2006·Parasite Immunology·M J de VeerE N T Meeusen
Jun 10, 2014·Parasite Immunology·F TamarozziM J Taylor
Jul 26, 2014·Immunology·Manisha PathakShailja Misra-Bhattacharya
Jul 21, 2007·Mediators of Inflammation·Chantima PorksakornAlan L Scott
Aug 11, 2016·PloS One·Nastaran MohammadiCarmelo Biondo
Oct 19, 2016·Scientific Reports·Francesca TamarozziMark J Taylor
Dec 23, 2016·Scientific Reports·Jesuthas AjendraMarc P Hübner
Oct 23, 2015·Journal of Leukocyte Biology·Pankaj SharmaMrigank Srivastava
Sep 18, 2013·Infection and Immunity·Tyler D ScherrTammy Kielian
May 15, 2019·Tropical Medicine and Infectious Disease·Emilio Rendón-FrancoAlvaro Aguilar Setién
Jul 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joseph D TurnerMark J Taylor
Jan 5, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy G HiseEric Pearlman
Nov 21, 2018·Brain Research Bulletin·An HotterbeekxRichard Idro