Wolbachia-induced neutrophil activation in a mouse model of ocular onchocerciasis (river blindness)

Infection and Immunity
I Gillette-FergusonEric Pearlman

Abstract

Endosymbiotic Wolbachia bacteria are abundant in the filarial nematodes that cause onchocerciasis (river blindness), including the larvae (microfilariae) that migrate into the cornea. Using a mouse model of ocular onchocerciasis, we recently demonstrated that it is these endosymbiotic bacteria rather than the nematodes per se that induce neutrophil infiltration to the corneal stroma and loss of corneal clarity (Saint Andre et al., Science 295:1892-1895, 2002). To better understand the role of Wolbachia organisms in the pathogenesis of this disease, we examined the fate of these bacteria in the cornea by immunoelectron microscopy. Microfilariae harboring Wolbachia organisms were injected into mouse corneas, and bacteria were detected with antibody to Wolbachia surface protein. Within 18 h of injection, neutrophils completely surrounded the nematodes and were in close proximity to Wolbachia organisms. Wolbachia surface protein labeling was also prominent in neutrophil phagosomes, indicating neutrophil ingestion of Wolbachia organisms. Furthermore, the presence of numerous electron-dense granules around the phagosomes indicated that neutrophils were activated. To determine if Wolbachia organisms directly activate neutrophils, peri...Continue Reading

References

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Citations

Jan 27, 2007·Current Infectious Disease Reports·Kelly L Johnston, Mark J Taylor
Mar 23, 2006·Infection and Immunity·Kristina H PorthouseThomas R Klei
Mar 29, 2008·Journal of Leukocyte Biology·Yan SunEric Pearlman
Jul 14, 2006·Emerging Infectious Diseases·Siobhán M O'ConnorJames M Hughes
Aug 27, 2010·Lancet·Mark J TaylorMoses Bockarie
Dec 26, 2006·Parasite Immunology·M J de VeerE N T Meeusen
Jun 10, 2014·Parasite Immunology·F TamarozziM J Taylor
Oct 19, 2016·Scientific Reports·Francesca TamarozziMark J Taylor
Jul 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joseph D TurnerMark J Taylor
Jan 5, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy G HiseEric Pearlman
Nov 21, 2018·Brain Research Bulletin·An HotterbeekxRichard Idro

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