PMID: 11607306Jul 15, 1992Paper

Wood contains a cell-wall structural protein

Proceedings of the National Academy of Sciences of the United States of America
W BaoR R Sederoff

Abstract

A pine extensin-like protein (PELP) has been localized in metabolically active cells of differentiating xylem and in mature wood of loblolly pine (Pinus taeda L.). This proline-rich glycosylated protein was purified from cell walls of differentiating xylem by differential solubility and gel electrophoresis. Polyclonal rabbit antibodies were raised against the deglycosylated purified protein (dPELP) and purified antibody was used for immunolocalization. Immunogold and alkaline phosphatase secondary antibody staining both show antigen in secondary cell walls of earlywood and less staining in latewood. Immunoassays of milled dry wood were developed and used to show increased availability of antigen after hydrogen fluoride or cellulase treatment and decreased antigen after chlorite treatment. The specificity of the antigen-antibody reaction was confirmed by competition assays and by preadsorption of antibody to the purified protein. We propose that extensin-like protein is present in xylem cell walls during lignification and that the protein remains as a structural component of cell walls in wood for many years after xylogenesis. We suggest that such structural proteins play important roles in the differentiation of xylem and there...Continue Reading

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Citations

Jan 1, 1995·Plant Molecular Biology·C A Loopstra, R R Sederoff
Jun 26, 2001·Phytochemistry·L A Donaldson
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Hiroo Fukuda
Jul 16, 2013·Plant Science : an International Journal of Experimental Plant Biology·Timothy J Strabala, Colleen P Macmillan
Oct 31, 2003·Journal of Experimental Botany·Sookyung OhKyung-Hwan Han
Mar 11, 2009·Anais Da Academia Brasileira De Ciências·Heber S AbreuKelysson F Amparado
Dec 1, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Komandoor Elayavalli AchyuthanAnup Kumar Singh
Feb 23, 2008·Organic & Biomolecular Chemistry·Mahelet Aweke TadesseFederica Sergi
Oct 1, 1993·The New Phytologist·Matilde Josè, Pere Puigdomènech

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