Wood formation from the base to the crown in Pinus radiata: gradients of tracheid wall thickness, wood density, radial growth rate and gene expression

Plant Molecular Biology
Sheree CatoRichard Gardner

Abstract

Wood formation was investigated at five heights along the bole for two unrelated trees of Pinus radiata. Both trees showed clear gradients in wood properties from the base to the crown. Cambial cells at the base of the tree were dividing 3.3-fold slower than those at the crown, while the average thickness of cell walls in wood was highest at the base. Cell wall thickness showed an overall correlation coefficient of >0.7 with wood density in both genotypes. Microscopic examination of developing tracheids showed that 33% of cells had formed secondary cell walls at the base of the tree, reducing to 3% at the crown. In total, 455 genes differentially expressed in developing xylem tissue from either the base or the crown were identified using modified differential display. RT-PCR analysis of 156 genes confirmed differential expression for 77%. Of the genes tested, 73% showed gradients in transcript abundance either up or down the bole of the tree, although the steepness of the gradients differed between genes. Genes involved in cell division and expansion tended to be more highly expressed in the crown of the tree, and two putative cell-cycle repressor genes were expressed 2-fold higher at the base. Conversely, transcripts of genes ...Continue Reading

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Citations

Jul 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Armin WagnerLana Te Kiri
Oct 31, 2008·Plant Physiology·Armin WagnerJohn Ralph
May 31, 2014·Tree Physiology·Gea GuerrieroJean-Francois Hausman
Feb 3, 2009·Current Opinion in Plant Biology·Dario GrattapagliaRonald R Sederoff
Jan 17, 2007·Trends in Plant Science·Taku Demura, Hiroo Fukuda
Feb 27, 2008·The New Phytologist·Jorge A P PaivaChristophe Plomion
Mar 24, 2011·The Plant Journal : for Cell and Molecular Biology·Armin WagnerJohn Ralph
Mar 13, 2014·Tree Physiology·Marcelo GarcésRaul Herrera
Nov 6, 2018·PloS One·Emily TelferLisa Stanbra

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