Wood Metabolomic Responses of Wild and Cultivated Grapevine to Infection with Neofusicoccum parvum , a Trunk Disease Pathogen

Metabolites
Clément LaboisJulie Chong

Abstract

Grapevine trunk diseases (GTDs), which are associated with complex of xylem-inhabiting fungi, represent one of the major threats to vineyard sustainability currently. Botryosphaeria dieback, one of the major GTDs, is associated with wood colonization by Botryosphaeriaceae fungi, especially Neofusicoccum parvum. We used GC-MS and HPLC-MS to compare the wood metabolomic responses of the susceptible Vitis vinifera subsp. vinifera (V.v. subsp. vinifera) and the tolerant Vitis vinifera subsp. sylvestris (V.v. subsp. sylvestris) after artificial inoculation with Neofusicoccum parvum (N. parvum). N. parvum inoculation triggered major changes in both primary and specialized metabolites in the wood. In both subspecies, infection resulted in a strong decrease in sugars (fructose, glucose, sucrose), whereas sugar alcohol content (mannitol and arabitol) was enhanced. Concerning amino acids, N. parvum early infection triggered a decrease in aspartic acid, serine, and asparagine, and a strong increase in alanine and -alanine. A trend for more intense primary metabolism alteration was observed in V.v. subsp.sylvestris compared to V. v. subsp.vinifera. N. parvum infection also triggered major changes in stilbene and flavonoid compounds. The c...Continue Reading

References

Feb 27, 2003·Biochemical and Biophysical Research Communications·Edna Ben-Izhak MonseliseDaniel Kost
Nov 24, 2004·Plant Physiology·Fatma KaplanCharles L Guy
Jun 10, 2006·BMC Genomics·Robert A van den BergMariët J van der Werf
Apr 11, 2007·The Journal of Cell Biology·Geoff CummingDavid L Vaux
Aug 17, 2010·Journal of Experimental Botany·Marta R M LimaAlberto C P Dias
Nov 14, 2012·Journal of Agricultural and Food Chemistry·Carole LambertStéphanie Cluzet
Jul 31, 2013·Frontiers in Plant Science·Remi LemoineMickael Durand
Aug 10, 2013·Journal of the Science of Food and Agriculture·Nassima ChaherTristan Richard
Jan 31, 2014·Environmental Science & Technology·Emma L SchymanskiJuliane Hollender
Sep 2, 2014·BMC Bioinformatics·Philippe BardouChristophe Klopp
Mar 10, 2015·Phytochemistry·Eliane Abou-MansourPhilippe Larignon
Apr 16, 2015·Journal of Experimental Botany·Dong DuanPeter Nick
May 20, 2015·Frontiers in Molecular Biosciences·Jun YangGuowang Xu
Oct 7, 2015·Frontiers in Microbiology·Mukesh MeenaRam S Upadhyay
Jan 1, 2017·Plant Physiology and Biochemistry : PPB·Marta R M LimaAlberto C P Dias
Jul 12, 2017·Journal of Agricultural and Food Chemistry·Denis RusjanMaja Mikulic-Petkovsek
Aug 26, 2017·Molecular Plant-microbe Interactions : MPMI·Maryline Magnin-RobertFlorence Fontaine
Jan 29, 2019·Frontiers in Plant Science·Florian MoretMarielle Adrian
Feb 11, 2019·Plant Cell Reports·Maryam SeifikalhorOksana Lastochkina
Jun 22, 2019·Journal of the Science of Food and Agriculture·Hong-Wei WangChuan-Chao Dai
Nov 23, 2019·Current Protocols in Bioinformatics·Jasmine ChongJianguo Xia

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear magnetic resonance
PCA
spore collection
feature extraction

Software Mentioned

Finder Natural Products Social Molecular Networking ( GNPS )
MetaboAnalyst
Agilent MassHunter
MS
GNPS
Excel
Agilent Profinder
jvenn
Finder

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.