Wood reinforcement of poplar by rice NAC transcription factor

Scientific Reports
Shingo SakamotoNobutaka Mitsuda

Abstract

Lignocellulose, composed of cellulose, hemicellulose, and lignin, in the secondary cell wall constitutes wood and is the most abundant form of biomass on Earth. Enhancement of wood accumulation may be an effective strategy to increase biomass as well as wood strength, but currently only limited research has been undertaken. Here, we demonstrated that OsSWN1, the orthologue of the rice NAC Secondary-wall Thickening factor (NST) transcription factor, effectively enhanced secondary cell wall formation in the Arabidopsis inflorescence stem and poplar (Populus tremula×Populus tremuloides) stem when expressed by the Arabidopsis NST3 promoter. Interestingly, in transgenic Arabidopsis and poplar, ectopic secondary cell wall deposition in the pith area was observed in addition to densification of the secondary cell wall in fiber cells. The cell wall content or density of the stem increased on average by up to 38% and 39% in Arabidopsis and poplar, respectively, without causing growth inhibition. As a result, physical strength of the stem increased by up to 57% in poplar. Collectively, these data suggest that the reinforcement of wood by NST3pro:OsSWN1 is a promising strategy to enhance wood-biomass production in dicotyledonous plant spe...Continue Reading

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Citations

Apr 30, 2017·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Hani Al-Ahmad
Mar 25, 2018·Physiologia Plantarum·Amir Mahboubi, Totte Niittylä
Jun 25, 2020·Physiologia Plantarum·Isura Sumeda Priyadarshana NagahageMasatoshi Yamaguchi
Sep 25, 2018·Plant Biotechnology·Isura Sumeda Priyadarshana NagahageMasatoshi Yamaguchi
Mar 30, 2018·Frontiers in Plant Science·Carolin SeyfferthHannele Tuominen
Oct 6, 2018·Nature Plants·Shingo SakamotoNobutaka Mitsuda

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Methods Mentioned

BETA
transgenic
transmission electron microscopy
fluorescence
Assay

Software Mentioned

ImageJ
Axioscop2 Plus

Related Concepts