Wool fibril sponges with perspective biomedical applications

Materials Science & Engineering. C, Materials for Biological Applications
A PatruccoC Tonin

Abstract

Sheep's wool was used as a natural source to prepare keratin microfibril sponges for scaffolding, by disruption of the histological structure of the fibres through mild alkali treatment, followed by ultrasonication, casting and salt-leaching. The wool sponges showed highly interconnected porosity (93%) and contain intrinsic sites of cellular recognition that mimic the extracellular matrix (ECM). They displayed good thermal and water stability due to the conversion of disulphide cystine bonds into shorter monosulphide lanthionine intermolecular bonds, but significantly swelled in water, because of the high hydrophilicity and porosity, with a volume increasing up to 38%. Nevertheless, sponges were stable in water without structural changes, with a neutral pH in aqueous media, and showed excellent resilience to repeated compression stresses. According to in vitro biocompatibility assays, wool fibril sponges showed a good cell adhesion and proliferation as proved by MTT, FDA assays and SEM observations. The unique structure of the cortical cell network made by wool keratin proteins with controlled-size macro-porosity suitable for cell guesting, and nutrient feeding, provides an excellent scaffold for future tissue engineering appli...Continue Reading

References

Sep 25, 1998·Journal of Biomaterials Science. Polymer Edition·K Y LeeI C Kwon
Dec 12, 2001·Journal of Biotechnology·Akira TachibanaKiyoshi Yamauchi
Jan 27, 2004·Biomaterials·Kazunori KatohKiyoshi Yamauchi
Sep 2, 2004·Biological & Pharmaceutical Bulletin·Toshihiro Fujii, Yusuke Ide
Oct 20, 2005·Journal of Bioscience and Bioengineering·Ayako KurimotoKiyoshi Yamauchi
Dec 26, 2006·Journal of Bioscience and Bioengineering·Akira TachibanaKiyoshi Yamauchi
Sep 19, 2008·Biomacromolecules·Marina ZoccolaMarco G Piacentino
Nov 28, 2008·Biomacromolecules·Andrew J PooleMickey G Huson
Feb 15, 2011·Biomaterials·Stephan ReichlGerd Geerling
May 2, 2012·Acta Biomaterialia·Andreia VasconcelosArtur Cavaco-Paulo
Mar 8, 2013·International Journal of Biological Macromolecules·Annalisa AluigiMaurizio Canetti
Apr 30, 2013·The Journal of Biological Chemistry·Qingchun ZhangGregory C Flynn
Oct 8, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Mira ParkHak-Yong Kim
Apr 15, 2014·Acta Biomaterialia·Bailey V Fearing, Mark E Van Dyke
Jun 9, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Ryo NakataToshizumi Tanabe

❮ Previous
Next ❯

Citations

Dec 28, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Ju WangBochu Wang
Oct 20, 2018·Frontiers in Bioengineering and Biotechnology·Giulia SuaratoAthanassia Athanassiou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Hoppe-Seyler's Zeitschrift für physiologische Chemie
A SCHOBERL, A WAGNER
Biochimica Et Biophysica Acta
S BLACKBURN, G R LEE
Current Drug Targets
Andreia Vasconcelos, Artur Cavaco-Paulo
Materials Science & Engineering. C, Materials for Biological Applications
Prachi KakkarB Madhan
© 2021 Meta ULC. All rights reserved