Workflow development for targeted lipidomic quantification using parallel reaction monitoring on a quadrupole-time of flight mass spectrometry

Analytica Chimica Acta
Juntuo ZhouYuxin Yin

Abstract

Advances in high-resolution mass spectrometers with faster scanning capabilities and higher sensitivities have expanded these instruments' functionality beyond traditional data-dependent acquisition in targeted metabolomics. Apart from the traditional multiple reaction monitoring strategy, the parallel reaction monitoring (PRM) strategy is also used for targeted metabolomics quantification. The high resolution and mass accuracy of full-scan (MS1) and tandem mass spectrometry (MS/MS) scan result in sufficient selectivity by monitoring all MS/MS fragment ions for each target precursor and simultaneously providing flexibility in assay method construction and post-acquisition data analysis. In this study, using an orthogonal quadrupole-time of flight liquid chromatography-mass spectrometry system (QTOF LC-MS), we investigated the applicability of a large-scale targeted lipidomic assay using scheduled PRM. This method monitored 222 lipids belonging to 15 lipid species in serum. Robustness, reproducibility, and quantitative performance were assessed using chemical standards and serum samples. Finally, we demonstrated the application of this PRM-based targeted metabolomic workflow to systemic lupus erythematosus, a severe autoimmunolo...Continue Reading

Citations

Jun 9, 2019·Metabolites·Farhana R PinuJacob Jaine
Dec 18, 2019·Bioanalysis·Karen SegersAnn Van Eeckhaut
Nov 17, 2019·Journal of Lipid Research·Alexander TrieblMarkus R Wenk
Dec 30, 2017·Journal of Mass Spectrometry : JMS·Marko Haramija
May 28, 2019·Frontiers in Immunology·Qiong ZhangYurong Qiu
Jun 16, 2019·Analytical and Bioanalytical Chemistry·Michel RaetzGérard Hopfgartner
Dec 29, 2019·Scientific Reports·Tejia ZhangRandall T Peterson
Jun 23, 2019·Metabolomics : Official Journal of the Metabolomic Society·Juntuo ZhouMing Cui
Jan 26, 2021·Journal of Lipid Research·Alexander TrieblMarkus R Wenk
Aug 2, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Bernd ReichlWolfgang Buchberger
Jun 3, 2021·Metabolites·Pauline Le FaouderJustine Bertrand-Michel
May 16, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Olga Angeliki BegouHelen G Gika

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