Worlds Apart - Transcriptome Profiles of Key Oral Microbes in the Periodontal Pocket Compared to Single Laboratory Culture Reflect Synergistic Interactions

Frontiers in Microbiology
Zhi-Luo DengIrene Wagner-Döbler

Abstract

Periodontitis is a worldwide prevalent oral disease which results from dysbiosis of the periodontal microbiome. Some of the most active microbial players, e.g.,Porphyromonas gingivalis, Treponema denticola, andFusobacterium nucleatum, have extensively been studied in the laboratory, but it is unclear to which extend these findings can be transferred toin vivoconditions. Here we show that the transcriptional profiles ofP. gingivalis, T. denticola, andF. nucleatumin the periodontal niche are distinct from those in single laboratory culture and exhibit functional similarities. GO (gene ontology) term enrichment analysis showed up-regulation of transporters, pathogenicity related traits and hemin/heme uptake mechanisms for all three speciesin vivo. Differential gene expression analysis revealed that cysteine proteases, transporters and hemin/heme-binding proteins were highly up-regulated in the periodontal niche, while genes involved in DNA modification were down-regulated. The data suggest strong interactions between those three species regarding protein degradation, iron up-take, and mobilityin vivo, explaining their enhanced synergistic pathogenicity. We discovered a strikingly high frequency of Single Nucleotide Polymorphisms (...Continue Reading

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Citations

Jul 30, 2019·Journal of Dental Research·D P MillerR J Lamont
Jun 14, 2020·International Journal of Molecular Sciences·Paulina ŚlęzakTeresa Olczak
Nov 14, 2018·Frontiers in Cellular and Infection Microbiology·Mark BonnerJulien Santi-Rocca
May 3, 2019·Mediators of Inflammation·Anilei HoareDenisse Bravo
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Nov 24, 2020·Periodontology 2000·Magda FeresFlavia Teles
Mar 7, 2021·International Journal of Molecular Sciences·Klaudia SiemińskaTeresa Olczak
Mar 11, 2021·Periodontology 2000·Matsuo Yamamoto, Ryo Aizawa

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Datasets Mentioned

BETA
PRJEB23061

Methods Mentioned

BETA
RNA-seq
electrophoresis
restriction modification
Illumina sequencing

Software Mentioned

SortMeRNA
edgeR
BWA
Fastq
VCFtools
R package topGO
Kraken
Mcf
BEDtools
BBMAP

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