PMID: 8967441May 1, 1996Paper

Wortmannin inhibits contraction without altering electrical activity in canine gastric smooth muscle

The American Journal of Physiology
E P BurkeN G Publicover

Abstract

Wortmannin, an inhibitor of myosin light-chain kinase (10-30 microM), completely and irreversibly abolished (in 75% of tissues from canine gastric antrum) phase contractions caused by slow waves with no significant effects on resting membrane potential or the frequency, amplitude, or duration of spontaneous slow waves. Responses to agents that normally cause hyperpolarization (cromakalim, sodium nitroprusside, and forskolin) were unaffected by wortmannin treatment. It was also possible to study the excitatory effects of agents and conditions that normally result in loss of intracellular impalements: 1) elevated extracellular K+ concentrations altered membrane potential close to values predicted by the Nernst equation, and 2) high concentrations of acetylcholine produced depolarization and rapid oscillations in membrane potential coincident with contractile activity. Cholinergic increases in myosin light-chain phosphorylation and contractions were partially blocked by wortmannin. In canine antrum, wortmannin inhibition of contraction was irreversible, although in other tissue types, partial recovery of contractions was observed when wortmannin was removed. Wortmannin can be a useful agent to investigate the electrophysiology of ...Continue Reading

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Citations

Aug 6, 1998·British Journal of Pharmacology·S J Hong, C C Chang
Aug 29, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·J M HemmingG M Mawe
May 9, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·A M FarrellyK D Keef
Sep 29, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·O BayguinovK M Sanders
Feb 23, 2019·The Journal of Experimental Biology·William JoyceTobias Wang
Nov 27, 2019·Biology of Reproduction·Xiaofeng MaSarah K England
Sep 28, 1999·British Journal of Pharmacology·S J Hong, C C Chang
Nov 15, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Edward ParrGary M Mawe
Nov 15, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A M DuffyK D Keef
Jun 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yong Zhang, William G Paterson
Aug 7, 2012·British Journal of Pharmacology·Hikaru HashitaniKeiichiro Nakamura
Dec 17, 2008·Animal Reproduction Science·S KupittayanantC Suwannachat

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