Wortmannin inhibits store-mediated calcium entry and protein tyrosine phosphorylation in human platelets

FEBS Letters
S JennerS O Sage

Abstract

The effects of the WT on store-mediated Ca2+ entry and protein tyrosine phosphorylation were investigated in fura-2-loaded human platelets. Wortmannin (2 microM) attenuated the rise in [Ca2+]i caused by Ca2+ entry while having no effect on the mobilisation of Ca2+ from internal stores. It also reduced store-depletion-evoked protein tyrosine phosphorylation. These findings demonstrate that WT is an inhibitor of tyrosine phosphorylation and store-mediated calcium entry and provide further evidence for the involvement of a tyrosine phosphorylation step in the link between Ca2+ store depletion and Ca2+ influx in human platelets.

References

Nov 11, 1990·Cell Calcium·J W Putney
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Dec 16, 1993·Biochimica Et Biophysica Acta·Y HashimotoK Kurokawa

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Citations

May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y ChoquetF A Wollman
Jun 15, 1997·The Journal of Physiology·B SomasundaramM P Mahaut-Smith
Jul 4, 2003·British Journal of Pharmacology·R FlemmingD J Beech
Mar 29, 2000·The Journal of Biological Chemistry·J A Rosado, S O Sage

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