Wound Healing Coordinates Actin Architectures to Regulate Mechanical Work

Nature Physics
Visar AjetiMichael P Murrell

Abstract

How cells with diverse morphologies and cytoskeletal architectures modulate their mechanical behaviors to drive robust collective motion within tissues is poorly understood. During wound repair within epithelial monolayers in vitro, cells coordinate the assembly of branched and bundled actin networks to regulate the total mechanical work produced by collective cell motion. Using traction force microscopy, we show that the balance of actin network architectures optimizes the wound closure rate and the magnitude of the mechanical work. These values are constrained by the effective power exerted by the monolayer, which is conserved and independent of actin architectures. Using a cell-based physical model, we show that the rate at which mechanical work is done by the monolayer is limited by the transformation between actin network architectures and differential regulation of cell-substrate friction. These results and our proposed mechanisms provide a robust physical model for how cells collectively coordinate their non-equilibrium behaviors to dynamically regulate tissue-scale mechanical output.

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Citations

Oct 4, 2020·The European Physical Journal. E, Soft Matter·Robert ChojowskiFalko Ziebert
Oct 3, 2018·PLoS Computational Biology·Michael F StaddonShiladitya Banerjee
Mar 14, 2021·Scientific Reports·Marie VersaevelSylvain Gabriele
May 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Eirini ManiouGabriel L Galea
Aug 17, 2021·Advanced Functional Materials·Alan Pasha TabatabaiMichael P Murrell
Aug 21, 2021·Physical Review. E·G M CarvalhoP Patrício
Oct 15, 2021·Advanced Materials·Erik MailandMahmut Selman Sakar

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Methods Mentioned

BETA
transfection
force measurement
force measurements

Software Mentioned

LifeAct
Fiji
MATLAB
Imaris
ImageJ
StackReg

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