Wound healing on athymic mice with engineered skin substitutes fabricated with keratinocytes harvested from an automated bioreactor.

The Journal of Surgical Research
Balaji Kalyanaraman, Steven T Boyce

Abstract

The Kerator is a computer controlled bioreactor for the automated culture and harvest of keratinocytes that can reduce labor and materials involved in the fabrication of engineered skin substitutes (ESS). Previous studies have shown that the Kerator is comparable to tissue culture flasks by keratinocyte confluence during culture, clonogenic potential of harvested keratinocytes and microanatomy, cell viability, and surface hydration of ESS fabricated with the harvested keratinocytes. In this study, the Kerator and tissue culture flasks were further compared by keratinocyte proliferation in vitro and wound healing after transplantation of ESS to athymic mice. The number of bromodeoxyuridine-positive keratinocytes in ESS fabricated with keratinocytes harvested from Kerator after 2 wk of in vitro maturation was 34 +/- 3 per high power field (hpf) (mean +/- SEM), which was not significantly different from that fabricated with keratinocytes harvested from flasks (34 +/- 1.5 per hpf). Percentage original wound area 6 wk after surgery of ESS fabricated with keratinocytes from the Kerator was 36% +/- 3.3%, which was not significantly different from that of ESS fabricated with keratinocytes from flasks (30% +/- 4.3%). In both cases, 78% ...Continue Reading

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Citations

Jan 1, 2014·Advanced Drug Delivery Reviews·Stephanie H MathesUrsula Graf-Hausner
Jun 22, 2012·Burns : Journal of the International Society for Burn Injuries·Stefanie MichaelPeter M Vogt
Mar 30, 2016·The Journal of Surgical Research·Paul WurzerLars P Kamolz
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Jun 19, 2021·Npj Regenerative Medicine·Álvaro Sierra-SánchezSalvador Arias-Santiago
Jul 9, 2021·International Wound Journal·Gabrielle NotorgiacomoStacey C Schutte

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